Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis

Raymond Konger, Zhengbin Xu, Ravi P. Sahu, Young L. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate that a spatial and temporal analysis of subclinical hyperemia reliably predicts specific areas at high risk for skin tumor development during photocarcinogenesis. To determine detailed spatiotemporal patterns of inflammatory angiogenesis foci in a relatively large area, we developed a mesoscopic (between microscopic and macroscopic) imaging approach. This method relies on our earlier finding that the combination of a spectral analysis of hemoglobin with diffuse-light-suppressed imaging can increase the image resolution, contrast and penetration depth to visualize microvasculature Hgb content in the large tissue area. In our recent study, SKH1 hairless albino mice were irradiated for 10 weeks with a carcinogen dose of UVB. Using our newly developed mesoscopic imaging methods, we imaged the mice over 20 - 30 weeks after stopping UVB, and excised hyperemic/non-hyperemic areas at several different timepoints. We show that persistent hyperemic foci can predict future tumor formation. In particular, our imaging approach allows us to assess the spatial and temporal extent of subclinical inflammatory foci, which in turn can predict sites of future overlying tumor formation. In addition, although COX-2 inhibitors are known to suppress skin cancer development in humans, it remains unclear whether the chemopreventive activity of COX-2 inhibitors are chiefly attributable to their anti-inflammatory effects. Our study provides evidence that subclinical subepithelial inflammatory foci occur prior to overt tumor formation, and that these areas are highly predictive for future tumor formation, that celecoxiba's ability to suppress tumorigenesis is tightly linked to its ability to reduce the area of subclinical inflammatory foci.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8926
ISBN (Print)9780819498397
DOIs
StatePublished - 2014
EventPhotonic Therapeutics and Diagnostics X - San Francisco, CA, United States
Duration: Feb 1 2014Feb 2 2014

Other

OtherPhotonic Therapeutics and Diagnostics X
CountryUnited States
CitySan Francisco, CA
Period2/1/142/2/14

Fingerprint

Hyperemia
Tumors
tumors
Imaging techniques
predictions
Aptitude
Cyclooxygenase 2 Inhibitors
Neoplasms
Skin
inhibitors
mice
Spatio-Temporal Analysis
Hairless Mouse
Carcinogens
carcinogens
Hemoglobin
Human Development
Skin Neoplasms
angiogenesis
Image resolution

Keywords

  • Chemoprevention
  • Hyperemia
  • Mesoscopic imaging
  • Photocarcinogenesis
  • Skin cancer

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Konger, R., Xu, Z., Sahu, R. P., & Kim, Y. L. (2014). Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8926). [89260Z] SPIE. https://doi.org/10.1117/12.2040891

Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis. / Konger, Raymond; Xu, Zhengbin; Sahu, Ravi P.; Kim, Young L.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8926 SPIE, 2014. 89260Z.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Konger, R, Xu, Z, Sahu, RP & Kim, YL 2014, Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8926, 89260Z, SPIE, Photonic Therapeutics and Diagnostics X, San Francisco, CA, United States, 2/1/14. https://doi.org/10.1117/12.2040891
Konger R, Xu Z, Sahu RP, Kim YL. Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8926. SPIE. 2014. 89260Z https://doi.org/10.1117/12.2040891
Konger, Raymond ; Xu, Zhengbin ; Sahu, Ravi P. ; Kim, Young L. / Tumor site prediction using spatiotemporal detection of subclinical hyperemia in experimental photocarcinogenesis. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8926 SPIE, 2014.
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