Tumor suppressor gene 14-3-3σ is down-regulated whereas the proto-oncogene translation elongation factor 1δ is up-regulated in non-small cell lung cancers as identified by proteomic profiling

Yang Liu, Qun Chen, Jian Ting Zhang

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Lung cancer, a leading cause of cancer deaths, consists of two major groups: small cell lung cancer (SCLC) and nonsmall cell lung cancer (NSCLC) with the NSCLC accounting for ∼75% cases of lung cancers. It has been suggested that molecular changes including overexpression of oncogenes and decreased expression of tumor suppressor genes are responsible for lung carcinogenesis. In this study, we analyzed protein profiles of four different human NSCLC cell lines compared with normal human bronchial epithelial cells using two-dimensional PAGE and MALDI-TOF mass spectrometry. We identified 12 protein spots with different expressions between the normal and cancer cells. Of these proteins, vimentin, cytokeratin 8, YB-1, PCNA, Nm23, hnRNP A2/B1, and HSP90β were known to be up-regulated in lung cancers, which is consistent with the current study. We also found that the expression of M-type pyruvate kinase is altered in NSCLC likely due to changes in translational control and/or differential phosphorylation of the protein. Interestingly, the expression of the tumor suppressor gene 14-3-3σ is down-regulated while that of the proto-oncogene TEF1δ is up-regulated in NSCLC cells. On the basis of these observations and previous studies, we propose that the altered expression of 14-3-3σ and TEF1δ may be involved in lung carcinogenesis.

Original languageEnglish (US)
Pages (from-to)728-735
Number of pages8
JournalJournal of Proteome Research
Volume3
Issue number4
DOIs
StatePublished - Jul 1 2004

    Fingerprint

Keywords

  • 14-3-3σ
  • Lung cancer
  • MALDI-TOF
  • Proteomics
  • Pyruvate kinase
  • TEF1δ

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this