Tumour budding, poorly differentiated clusters, and T-cell response in colorectal cancer

Kenji Fujiyoshi, Juha P. Väyrynen, Jennifer Borowsky, David J. Papke, Kota Arima, Koichiro Haruki, Junko Kishikawa, Naohiko Akimoto, Tomotaka Ugai, Mai Chan Lau, Simeng Gu, Shanshan Shi, Melissa Zhao, Annacarolina Fabiana Lucia Da Silva, Tyler S. Twombly, Hongmei Nan, Jeffrey A. Meyerhardt, Mingyang Song, Xuehong Zhang, Kana WuAndrew T. Chan, Charles S. Fuchs, Jochen K. Lennerz, Marios Giannakis, Jonathan A. Nowak, Shuji Ogino

Research output: Contribution to journalArticle

Abstract

Background: Tumour budding and poorly differentiated clusters (PDC) represent forms of tumour invasion. We hypothesised that T-cell densities (reflecting adaptive anti-tumour immunity) might be inversely associated with tumour budding and PDC in colorectal carcinoma. Methods: Utilising 915 colon and rectal carcinomas in two U.S.-wide prospective cohort studies, and multiplex immunofluorescence combined with machine learning algorithms, we assessed CD3, CD4, CD8, CD45RO (PTPRC), and FOXP3 co-expression patterns in lymphocytes. Tumour budding and PDC at invasive fronts were quantified by digital pathology and image analysis using the International tumour Budding Consensus Conference criteria. Using covariate data of 4,420 incident colorectal cancer cases, inverse probability weighting (IPW) was integrated with multivariable logistic regression analysis that assessed the association of T-cell subset densities with tumour budding and PDC while adjusting for selection bias due to tissue availability and potential confounders, including microsatellite instability status. Findings: Tumour budding counts were inversely associated with density of CD3+CD8+ [lowest vs. highest: multivariable odds ratio (OR), 0.50; 95% confidence interval (CI), 0.35–0.70; Ptrend < 0.001] and CD3+CD8+CD45RO+ cells (lowest vs. highest: multivariable OR, 0.44; 95% CI, 0.31–0.63; Ptrend < 0.001) in tumour epithelial region. Tumour budding levels were associated with higher colorectal cancer-specific mortality (multivariable hazard ratio, 2.13; 95% CI, 1.57–2.89; Ptrend < 0.001) in Cox regression analysis. There were no significant associations of PDC with T-cell subsets. Interpretation: Tumour epithelial naïve and memory cytotoxic T cell densities are inversely associated with tumour budding at invasive fronts, suggesting that cytotoxic anti-tumour immunity suppresses tumour microinvasion.

Original languageEnglish (US)
Article number102860
JournalEBioMedicine
Volume57
DOIs
StatePublished - Jul 2020
Externally publishedYes

Keywords

  • adenocarcinoma
  • artificial intelligence, clinical outcomes
  • epithelial mesenchymal transition
  • host-tumour interaction
  • molecular pathological epidemiology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Fujiyoshi, K., Väyrynen, J. P., Borowsky, J., Papke, D. J., Arima, K., Haruki, K., Kishikawa, J., Akimoto, N., Ugai, T., Lau, M. C., Gu, S., Shi, S., Zhao, M., Da Silva, A. F. L., Twombly, T. S., Nan, H., Meyerhardt, J. A., Song, M., Zhang, X., ... Ogino, S. (2020). Tumour budding, poorly differentiated clusters, and T-cell response in colorectal cancer. EBioMedicine, 57, [102860]. https://doi.org/10.1016/j.ebiom.2020.102860