Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control

Paula Ventura da Silveira, Beatriz Helena Dias Panariello, Cecília Atem Gonçalves de Araújo Costa, Shawn M. Maule, Shane M. Maule, Malvin N. Janal, Iriana Carla Junqueira Zanin, Simone Duarte

Research output: Contribution to journalArticle

Abstract

Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm−2 for blue light and 43.8 J cm2, 87.6 J cm2, and 175.5 J cm2 for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12% chlorhexidine (CHX) and 0.89% NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p < 0.001) and approached PC levels with both red and blue light treatments. CFU were also lower in groups exposed to blue light and higher durations of red light (p < 0.05). EPS-soluble and EPS-insoluble measures were variably reduced by these light exposures. In conclusion, twice-daily exposure to both blue and red lights affect the biofilm development and physiology of polysaccharide production and are potential mechanisms for the control of C. albicans biofilm matrix development.

Original languageEnglish (US)
JournalLasers in Medical Science
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

Biofilms
Candida albicans
Light
Therapeutics
Phototherapy
Stem Cells
Weights and Measures
Chlorhexidine
Photosensitizing Agents
Bacterial Infections
Polysaccharides
Analysis of Variance
Control Groups

Keywords

  • Biofilm
  • Blue light
  • Candida albicans
  • Phototherapy
  • Red light

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

da Silveira, P. V., Panariello, B. H. D., de Araújo Costa, C. A. G., Maule, S. M., Maule, S. M., Janal, M. N., ... Duarte, S. (Accepted/In press). Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control. Lasers in Medical Science. https://doi.org/10.1007/s10103-018-2610-x

Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control. / da Silveira, Paula Ventura; Panariello, Beatriz Helena Dias; de Araújo Costa, Cecília Atem Gonçalves; Maule, Shawn M.; Maule, Shane M.; Janal, Malvin N.; Zanin, Iriana Carla Junqueira; Duarte, Simone.

In: Lasers in Medical Science, 01.01.2018.

Research output: Contribution to journalArticle

da Silveira, Paula Ventura ; Panariello, Beatriz Helena Dias ; de Araújo Costa, Cecília Atem Gonçalves ; Maule, Shawn M. ; Maule, Shane M. ; Janal, Malvin N. ; Zanin, Iriana Carla Junqueira ; Duarte, Simone. / Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control. In: Lasers in Medical Science. 2018.
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abstract = "Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm−2 for blue light and 43.8 J cm2, 87.6 J cm2, and 175.5 J cm2 for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12{\%} chlorhexidine (CHX) and 0.89{\%} NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p < 0.001) and approached PC levels with both red and blue light treatments. CFU were also lower in groups exposed to blue light and higher durations of red light (p < 0.05). EPS-soluble and EPS-insoluble measures were variably reduced by these light exposures. In conclusion, twice-daily exposure to both blue and red lights affect the biofilm development and physiology of polysaccharide production and are potential mechanisms for the control of C. albicans biofilm matrix development.",
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AU - Maule, Shawn M.

AU - Maule, Shane M.

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AB - Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm−2 for blue light and 43.8 J cm2, 87.6 J cm2, and 175.5 J cm2 for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12% chlorhexidine (CHX) and 0.89% NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p < 0.001) and approached PC levels with both red and blue light treatments. CFU were also lower in groups exposed to blue light and higher durations of red light (p < 0.05). EPS-soluble and EPS-insoluble measures were variably reduced by these light exposures. In conclusion, twice-daily exposure to both blue and red lights affect the biofilm development and physiology of polysaccharide production and are potential mechanisms for the control of C. albicans biofilm matrix development.

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