Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles

Momoko Yoshimoto, Hsi Chang, Mitsutaka Shiota, Hirohiko Kobayashi, Katsutsugu Umeda, Atsushi Kawakami, Toshio Heike, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recent studies have indicated that bone marrow cells can regenerate damaged muscles and that they can adopt phenotypes of other cells by cell fusion. Our direct visualization system gave evidence of massive muscle regeneration by green fluorescent protein (GFP)-labeled CD45+c-Kit +Sca-1+Lin- cells (KSL cells), and we investigated the role of KSL cells in muscle regeneration after transplantation with or without lethal irradiation. In the early phase, GFP signals were clearly observed in all the muscles of only irradiated mice. Transverse cryostat sections showed GFP+myosin+ muscle fibers, along with numerous GFP+ hematopoietic cells in damaged muscle. These phenomena were temporary, and GFP signals had dramatically reduced 30 days after transplantation. After 6 months, GFP+ fibers could hardly be detected, but GFP+c-Met+ mononuclear cells were located beneath the basal lamina where satellite cells usually exist in both conditioned mice. Immunostaining of isolated single fibers revealed GFP +PAX7+, GFP+MyoD+, and GFP +Myf5+ satellite-like cells on the fibers. Single-fiber cultures from these mice showed proliferation of GFP+ fibers. These results indicate two different roles of KSL cells: one leading to regeneration of damaged muscles in the early phase and the other to conversion into satellite cells in the late phase.

Original languageEnglish (US)
Pages (from-to)610-618
Number of pages9
JournalStem Cells
Volume23
Issue number5
DOIs
StatePublished - May 2005
Externally publishedYes

Fingerprint

Cell Transplantation
Green Fluorescent Proteins
Muscles
Regeneration
Transplantation
Cell Fusion
Myosins
Basement Membrane
Bone Marrow Cells
Muscle Cells
Phenotype

Keywords

  • c-KitSca-1 Lin
  • Hematopoietic stem cells
  • Muscle stem cells
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Yoshimoto, M., Chang, H., Shiota, M., Kobayashi, H., Umeda, K., Kawakami, A., ... Nakahata, T. (2005). Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles. Stem Cells, 23(5), 610-618. https://doi.org/10.1634/stemcells.2004-0220

Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles. / Yoshimoto, Momoko; Chang, Hsi; Shiota, Mitsutaka; Kobayashi, Hirohiko; Umeda, Katsutsugu; Kawakami, Atsushi; Heike, Toshio; Nakahata, Tatsutoshi.

In: Stem Cells, Vol. 23, No. 5, 05.2005, p. 610-618.

Research output: Contribution to journalArticle

Yoshimoto, M, Chang, H, Shiota, M, Kobayashi, H, Umeda, K, Kawakami, A, Heike, T & Nakahata, T 2005, 'Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles', Stem Cells, vol. 23, no. 5, pp. 610-618. https://doi.org/10.1634/stemcells.2004-0220
Yoshimoto M, Chang H, Shiota M, Kobayashi H, Umeda K, Kawakami A et al. Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles. Stem Cells. 2005 May;23(5):610-618. https://doi.org/10.1634/stemcells.2004-0220
Yoshimoto, Momoko ; Chang, Hsi ; Shiota, Mitsutaka ; Kobayashi, Hirohiko ; Umeda, Katsutsugu ; Kawakami, Atsushi ; Heike, Toshio ; Nakahata, Tatsutoshi. / Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles. In: Stem Cells. 2005 ; Vol. 23, No. 5. pp. 610-618.
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