Two-dimensional electrophoretic mapping of hepatic and renal stress proteins

Frank Witzmann, J. Clack, C. Fultz, B. Jarnot

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cellular stress proteins and molecular chaperones are responsive to a variety of stressors and therefore comprise an ideal set of proteins with the potential to be used as biomarkers of chemical toxicity. We have investigated the expression of a group of well established heat shock and glucose-regulated proteins in the rat liver and kidney using large-scale two-dimensional protein electrophoresis and computerized image analysis. Our goal was to determine the level of their expression in unstressed target tissues and map their coordinate positions on conventional format two-dimensional electrophoresis (2-DE) gels. All the proteins studied, except for Hsp25 (heat-shock protein) whose expression fell below the level of analyzable detection, were constitutively expressed in liver and kidney. With the exception of Hsp70, all the stress proteins analyzed were constitutively more abundant in the liver than the kidney. Comparison of the sum total of all stress protein abundances revealed a nearly threefold higher level of expression in the liver than the kidney. Our results suggest that this group of proteins has significant responsibilities in normal, unstressed cells, due to their constitutive abundance. Correspondingly, the 2-DE stress protein pattern established in this study may be very useful in toxicologic screening as well as describing a broad range of molecular effects of xenobiotic exposure.

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalElectrophoresis
Volume16
Issue number3
StatePublished - 1995

Fingerprint

Heat-Shock Proteins
Liver
Kidney
Electrophoresis
Proteins
Molecular Chaperones
Biomarkers
Xenobiotics
Image analysis
Toxicity
Rats
Shock
Screening
Hot Temperature
Gels
Tissue

Keywords

  • heat-shock
  • kidney
  • liver
  • protein mapping
  • stress proteins
  • two-dimensional electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Two-dimensional electrophoretic mapping of hepatic and renal stress proteins. / Witzmann, Frank; Clack, J.; Fultz, C.; Jarnot, B.

In: Electrophoresis, Vol. 16, No. 3, 1995, p. 451-459.

Research output: Contribution to journalArticle

Witzmann, F, Clack, J, Fultz, C & Jarnot, B 1995, 'Two-dimensional electrophoretic mapping of hepatic and renal stress proteins', Electrophoresis, vol. 16, no. 3, pp. 451-459.
Witzmann, Frank ; Clack, J. ; Fultz, C. ; Jarnot, B. / Two-dimensional electrophoretic mapping of hepatic and renal stress proteins. In: Electrophoresis. 1995 ; Vol. 16, No. 3. pp. 451-459.
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