Two-dimensional gels for toxicological drug discovery applications

Frank A. Witzmann, Matthew R. Richardson

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Two-dimensional gel electrophoresis (2DGE) continues to be a useful approach to study protein expression. Although liquid chromatographic and mass spectrometric approaches that overcome some of the limitations and labour intensity of 2DGE are increasingly popular, this electrophoretic approach still has exceptional relevance in toxicology. Despite the technical challenges, pharmacologists/toxicologists continue to use gel-based proteomics to assess the biological and health effects of chemical treatment and exposure. This brief review addresses the use of 2DGE-based proteomics in drug development and toxicology, emphasising its unique strengths and weaknesses, and considers recent developments in this strategy that have evolved to directly confront the issues of dynamic range and reproducibility that have previously limited the overall use of 2D electrophoresis.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalExpert Opinion on Drug Metabolism and Toxicology
Volume2
Issue number1
DOIs
StatePublished - Dec 1 2006

Fingerprint

Drug Discovery
Electrophoresis
Proteomics
Toxicology
Gels
Electrophoresis, Gel, Two-Dimensional
Health
Personnel
Liquids
Pharmaceutical Preparations
Proteins

Keywords

  • Complexity reduction
  • Differential centrifugation
  • Isoelectric focusing
  • Mass spectrometry
  • Proteomics
  • Sample fractionation
  • Toxicology
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

Two-dimensional gels for toxicological drug discovery applications. / Witzmann, Frank A.; Richardson, Matthew R.

In: Expert Opinion on Drug Metabolism and Toxicology, Vol. 2, No. 1, 01.12.2006, p. 103-111.

Research output: Contribution to journalReview article

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