Two pathways for tBID-induced cytochrome c release from rat brain mitochondria: BAK- versus BAX-dependence

Nikolai Broustovetski, Janet M. Dubinsky, Bruno Antonssont, Ronald Jemmerson

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The mechanisms of truncated BID (tBID)-induced Cyt c release from non-synaptosomal brain mitochondria were examined. Addition of tBID to mitochondria induced partial Cyt c release which was inhibited by anti-BAK antibodies, implicating BAK. Immunoblotting showed the presence of BAK, but not BAX, in brain mitochondria. tBID did not release Cyt c from rat liver mitochondria, which lacked both BAX and BAK. This indicated that tBID did not act independently of BAX and BAK. tBID plus monomeric BAX produced twice as much Cyt c release as did tBID or oligomeric BAX alone. Neither tBID alone nor in combination with BAX induced mitochondrial swelling. In both cases Cyt c was insensitive to cyclosporin A plus ADP, inhibitors of the mitochondrial permeability transition (mPT). Recombinant Bcl-xL inhibited Cyt c release induced by tBID alone or in combination with monomeric BAX. Koenig's polyanion, an inhibitor of VDAC, suppressed tBID-induced Cyt c release from brain mitochondria mediated by BAK but not by BAX. Thus, tBID can induce mPT-independent Cyt c release from brain mitochondria by interacting with exogenous BAX and/or with endogenous BAK that may involve VDAC. In contrast, neither adenylate kinase nor Smac/DIABLO was released from isolated rat brain mitochondria via BAK or BAX.

Original languageEnglish (US)
Pages (from-to)196-207
Number of pages12
JournalJournal of Neurochemistry
Volume84
Issue number1
DOIs
StatePublished - Jan 2003
Externally publishedYes

Fingerprint

Mitochondria
Cytochromes c
Rats
Brain
Permeability
Mitochondrial Swelling
Adenylate Kinase
Liver Mitochondrion
Immunoblotting
Adenosine Diphosphate
Cyclosporine
Anti-Idiotypic Antibodies
Liver
Swelling
Antibodies

Keywords

  • Apoptosis
  • BAK
  • BAX
  • Cytochrome c
  • Permeability transition
  • tBID

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Two pathways for tBID-induced cytochrome c release from rat brain mitochondria : BAK- versus BAX-dependence. / Broustovetski, Nikolai; Dubinsky, Janet M.; Antonssont, Bruno; Jemmerson, Ronald.

In: Journal of Neurochemistry, Vol. 84, No. 1, 01.2003, p. 196-207.

Research output: Contribution to journalArticle

Broustovetski, Nikolai ; Dubinsky, Janet M. ; Antonssont, Bruno ; Jemmerson, Ronald. / Two pathways for tBID-induced cytochrome c release from rat brain mitochondria : BAK- versus BAX-dependence. In: Journal of Neurochemistry. 2003 ; Vol. 84, No. 1. pp. 196-207.
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