Two-photon intravital imaging of thrombus development

Malgorzata Kamocka, Jian Mu, Xiaomin Liu, Nan Chen, Amy Zollman, Barbara Sturonas-Brown, Kenneth Dunn, Zhiliang Xu, Danny Z. Chen, Mark S. Alber, Elliot Rosen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Thrombus development in mouse mesenteric vessels following laser-induced injury was monitored by high-resolution, near-real-time, two-photon, intravital microscopy. In addition to the use of fluorescently tagged fibrin(ogen) and platelets, plasma was labeled with fluorescently tagged dextran. Because blood cells exclude the dextran in the single plane, blood cells appear as black silhouettes. Thus, in addition to monitoring the accumulation of platelets and fibrin in the thrombus, the protocol detects the movement and incorporation of unlabeled cells in and around it. The developing thrombus perturbs the blood flow near the thrombus surface, which affects the incorporation of platelets and blood cells into the structure. The hemodynamic effects and incorporation of blood cells lead to the development of thrombi with heterogeneous domain structures. Additionally, image processing algorithms and simulations were used to quantify structural features of developing thrombi. This analysis suggests a novel mechanism to stop the growth of developing thrombus.

Original languageEnglish
Article number016020
JournalJournal of Biomedical Optics
Volume15
Issue number1
DOIs
StatePublished - 2010

Fingerprint

blood cells
Blood
Photons
platelets
fibrin
Imaging techniques
Platelets
dextrans
Cells
photons
Dextran
Dextrans
Fibrin
hemodynamics
blood flow
cells
vessels
mice
image processing
Hemodynamics

Keywords

  • Coagulation
  • Hemostasis
  • Intravital microscopy
  • Laser vascular injury
  • Thrombogenesis
  • Two-photon imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Two-photon intravital imaging of thrombus development. / Kamocka, Malgorzata; Mu, Jian; Liu, Xiaomin; Chen, Nan; Zollman, Amy; Sturonas-Brown, Barbara; Dunn, Kenneth; Xu, Zhiliang; Chen, Danny Z.; Alber, Mark S.; Rosen, Elliot.

In: Journal of Biomedical Optics, Vol. 15, No. 1, 016020, 2010.

Research output: Contribution to journalArticle

Kamocka, M, Mu, J, Liu, X, Chen, N, Zollman, A, Sturonas-Brown, B, Dunn, K, Xu, Z, Chen, DZ, Alber, MS & Rosen, E 2010, 'Two-photon intravital imaging of thrombus development', Journal of Biomedical Optics, vol. 15, no. 1, 016020. https://doi.org/10.1117/1.3322676
Kamocka M, Mu J, Liu X, Chen N, Zollman A, Sturonas-Brown B et al. Two-photon intravital imaging of thrombus development. Journal of Biomedical Optics. 2010;15(1). 016020. https://doi.org/10.1117/1.3322676
Kamocka, Malgorzata ; Mu, Jian ; Liu, Xiaomin ; Chen, Nan ; Zollman, Amy ; Sturonas-Brown, Barbara ; Dunn, Kenneth ; Xu, Zhiliang ; Chen, Danny Z. ; Alber, Mark S. ; Rosen, Elliot. / Two-photon intravital imaging of thrombus development. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 1.
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