Two promoters mediate transcription from the human LHX3 gene: Involvement of nuclear factor I and specificity protein 1

Benjamin C. Yaden, Marin Garcia, Timothy P L Smith, Simon Rhodes

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The LHX3 transcription factor is required for pituitary and nervous system development in mammals. Mutations in the human gene are associated with hormone-deficiency diseases. The gene generates two mRNAs, hLHX3a and hLHX3b, which encode three proteins with different properties. Here, the cis elements and trans-acting factors that regulate the basal transcription of the two mRNAs are characterized. A comparative approach was taken featuring analysis of seven mammalian Lhx3 genes, with a focus on the human gene. Two conserved, TATA-less, GC-rich promoters that are used to transcribe the mRNAs precede exons 1a and 1b of hLHX3. Transcription start sites were mapped for both promoters. Deletion experiments showed most activity for reporter genes containing the basal promoters in the context of -2.0 kb of hLHX3a and 1.8 kb of intron 1a (hLHX3b). Transfection, site-directed mutation, electrophoretic mobility shift, Southwestern blot, and chromatin immunoprecipitation approaches were used to characterize the interaction of transcription factors with conserved elements in the promoters. Specificity protein 1 is a regulator of both promoters through interaction with GC boxes. In addition, a distal element within intron 1a that is recognized by nuclear factor I is critical for hLHX3b promoter function. We conclude that dual promoters allow regulated production of two hLHX3 mRNAs.

Original languageEnglish
Pages (from-to)324-337
Number of pages14
JournalEndocrinology
Volume147
Issue number1
DOIs
StatePublished - Jan 2006

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NFI Transcription Factors
Messenger RNA
Introns
Genes
Proteins
Southwestern Blotting
Transcription Factors
Deficiency Diseases
Mutation
Trans-Activators
Chromatin Immunoprecipitation
Transcription Initiation Site
Reporter Genes
Nervous System
Transfection
Mammals
Exons
Hormones

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Two promoters mediate transcription from the human LHX3 gene : Involvement of nuclear factor I and specificity protein 1. / Yaden, Benjamin C.; Garcia, Marin; Smith, Timothy P L; Rhodes, Simon.

In: Endocrinology, Vol. 147, No. 1, 01.2006, p. 324-337.

Research output: Contribution to journalArticle

Yaden, Benjamin C. ; Garcia, Marin ; Smith, Timothy P L ; Rhodes, Simon. / Two promoters mediate transcription from the human LHX3 gene : Involvement of nuclear factor I and specificity protein 1. In: Endocrinology. 2006 ; Vol. 147, No. 1. pp. 324-337.
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