Two signal transduction pathways mediate interleukin-1 receptor expression in Balb/c3T3 fibroblasts

Paul D. Bonin, William J. Chiou, James E. McGee, Jai Pal Singh

Research output: Contribution to journalArticle

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Abstract

Our previous studies showed that platelet-derived growth factor (PDGF) modulated interleukin-1 (IL-1) activity and IL-1 binding to Balb/c3T3 fibroblasts (Bonin, P. D., and Singh, J. P. (1988) J. Biol. Chem. 263, 11052-11055). Subsequent studies have demonstrated an action of PDGF at the level of IL-1 receptor (IL-1R) gene expression. PDGF treatment of Balb/ c3T3 cells produces a 10-20-fold stimulation of mRNA for IL-1 receptor. Investigation of the signal transduction pathways shows that activation of either the protein kinase C pathway or the cAMP-mediated pathway leads to the stimulation of IL-1 receptor expression in Balb/c3T3 cells. Treatment of Balb/c3T3 cells with phorbol 12-myristate 13-acetate (PMA), a known activator of protein kinase C, produced an increased 125I-IL-1 binding to cells and stimulation of IL-1R mRNA. Staurosporine, an inhibitor of protein kinase C, blocked the induction of IL-1 binding by PDGF or PMA. Down-regulation of protein kinase C by pretreatment with PMA reduced the subsequent stimulation by PDGF. Chronic treatment with PMA, however, did not produce a complete inhibition of PDGF effect on IL-1R. Further studies showed that the agents that stimulate cAMP accumulation (isobutyl methylxanthine, dibutyryl), directly stimulate adenylate cyclase (forskolin), or activate G protein (choleragen) stimulated 125I-IL-1 binding and IL-1R mRNA accumulation in Balb/c3T3 cells. These studies suggest that potentially two signal transduction pathways mediate IL-1 receptor expression in Balb/c3T3 fibroblasts. Evidence is presented that suggests that stimulation of IL-1R through these two pathways (PMA/PDGF-stimulated and cAMP-stimulated) occurs independent of each other.

Original languageEnglish (US)
Pages (from-to)18643-18649
Number of pages7
JournalJournal of Biological Chemistry
Volume265
Issue number30
StatePublished - Oct 25 1990
Externally publishedYes

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Signal transduction
Interleukin-1 Receptors
Fibroblasts
Platelet-Derived Growth Factor
Signal Transduction
Interleukin-1
Acetates
Protein Kinase C
Messenger RNA
Staurosporine
Cholera Toxin
Colforsin
GTP-Binding Proteins
Adenylyl Cyclases
Gene expression
Down-Regulation
Chemical activation
phorbol-12-myristate
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bonin, P. D., Chiou, W. J., McGee, J. E., & Singh, J. P. (1990). Two signal transduction pathways mediate interleukin-1 receptor expression in Balb/c3T3 fibroblasts. Journal of Biological Chemistry, 265(30), 18643-18649.

Two signal transduction pathways mediate interleukin-1 receptor expression in Balb/c3T3 fibroblasts. / Bonin, Paul D.; Chiou, William J.; McGee, James E.; Singh, Jai Pal.

In: Journal of Biological Chemistry, Vol. 265, No. 30, 25.10.1990, p. 18643-18649.

Research output: Contribution to journalArticle

Bonin, PD, Chiou, WJ, McGee, JE & Singh, JP 1990, 'Two signal transduction pathways mediate interleukin-1 receptor expression in Balb/c3T3 fibroblasts', Journal of Biological Chemistry, vol. 265, no. 30, pp. 18643-18649.
Bonin, Paul D. ; Chiou, William J. ; McGee, James E. ; Singh, Jai Pal. / Two signal transduction pathways mediate interleukin-1 receptor expression in Balb/c3T3 fibroblasts. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 30. pp. 18643-18649.
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