Two subcellular localizations of eIF3 p170 and its interaction with membrane-bound microfilaments: Implications for alternative functions of p170

Roxana Pincheira, Qun Chen, Zhisheng Huang, Jian-Ting Zhang

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29 Citations (Scopus)

Abstract

We previously identified a 170-kDa protein (p170) highly expressed in lung cancers as the major subunit of the eukaryotic translation initiation factor 3 (eIF3). p170 was recently cloned and little is known concerning its characteristics and subcellular localization. In this paper, we report our surprising findings that about 20% of p170 is associated with membranes while the remaining portion is located in the cytoplasm presumably in the eIF3 complex. We also find that p170 interacts with both endoplasmic reticulum and plasma membranes. The binding of p170 to membranes is through actin filaments, consistent with the fact that p170 contains a spectrin repeat motif that may be involved in actin binding. Furthermore, the cytoplasmic p170 is phosphorylated at serine and threonine residues and the phosphorylation is stimulated by serum. However, the membrane-actin-bound p170 is not phosphorylated. The results obtained in this study suggest that p170 may have other functions in addition to participating in translation initiation. Phosphorylation may play an important regulatory role in the function of p170 in translation initiation and other alternative functions.

Original languageEnglish
Pages (from-to)410-418
Number of pages9
JournalEuropean Journal of Cell Biology
Volume80
Issue number6
StatePublished - 2001

Fingerprint

Prokaryotic Initiation Factor-3
Eukaryotic Initiation Factor-3
Eukaryotic Initiation Factors
Actin Cytoskeleton
Membranes
Actins
Phosphorylation
Spectrin
Threonine
Endoplasmic Reticulum
Serine
Lung Neoplasms
Cytoplasm
Cell Membrane
Serum
Proteins

Keywords

  • Cell growth control
  • eIF3
  • Lung cancer
  • Membrane protein
  • P-glycoprotein
  • p170
  • Phosphorylation

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Two subcellular localizations of eIF3 p170 and its interaction with membrane-bound microfilaments : Implications for alternative functions of p170. / Pincheira, Roxana; Chen, Qun; Huang, Zhisheng; Zhang, Jian-Ting.

In: European Journal of Cell Biology, Vol. 80, No. 6, 2001, p. 410-418.

Research output: Contribution to journalArticle

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