Two types of ventricular fibrillation in isolated rabbit hearts

Importance of excitability and action potential duration restitution

Tsu Juey Wu, Shien-Fong Lin, James N. Weiss, Chih Tai Ting, Peng-Sheng Chen

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Background - The combined effects of excitability and action potential duration (APD) restitution on wavefront dynamics remain unclear. Methods and Results - We used optical mapping techniques to study Langendorff-perfused rabbit hearts. In protocol IA (n=10), D600 at increasing concentrations was infused during ventricular fibrillation (VF). With concentration increased to 0.5 mg/L, fast VF (dominant frequency, 19.1 ± 1.8 Hz) was consistently converted to ventricular tachycardia (VT). However, increasing D600 further to 2.5 or 5.0 mg/L converted VT to slow VF (11.9±2.3 Hz, P=0.0011). In an additional 4 hearts (protocol IB), tetrodotoxin converted a preexisting VT to slow VF (11.0±1.4 Hz). Optical maps show wandering wavelets in fast VF, organized reentry in VT, and spatiotemporal periodicity in slow VF. In protocol II, we determined APD and conduction time-1 (CT-1) restitutions during D600 infusion. CT-1 was used as an estimate of excitability. At 0.1 mg/L, APD and CT-1 restitutions were steep and flat, respectively. APD restitution became flattened when D600 increased to 0.5 mg/L, converting fast VF to VT. Further increasing D600 to 2.5 or 5.0 mg/L steepened CT-1 restitution and widened the range of S1 pacing cycle lengths over which CT-1 decreased, converting VT to slow VF. Conclusions - Two types of VF exist in isolated rabbit hearts. Fast (type I) VF is associated with a steep APD restitution, a flat CT-1 restitution, and wandering wavelets. Slow (type II) VF is associated with a flat APD restitution, a steep CT-1 restitution, and spatiotemporal periodicity. Both excitability and APD restitution are important in VF maintenance.

Original languageEnglish (US)
Pages (from-to)1859-1866
Number of pages8
JournalCirculation
Volume106
Issue number14
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Ventricular Fibrillation
Action Potentials
Rabbits
Gallopamil
Accelerated Idioventricular Rhythm
Ventricular Tachycardia
Periodicity
Tetrodotoxin
Maintenance

Keywords

  • Arrhythmia
  • Fibrillation
  • Mapping
  • Ventricles

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Two types of ventricular fibrillation in isolated rabbit hearts : Importance of excitability and action potential duration restitution. / Wu, Tsu Juey; Lin, Shien-Fong; Weiss, James N.; Ting, Chih Tai; Chen, Peng-Sheng.

In: Circulation, Vol. 106, No. 14, 01.10.2002, p. 1859-1866.

Research output: Contribution to journalArticle

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abstract = "Background - The combined effects of excitability and action potential duration (APD) restitution on wavefront dynamics remain unclear. Methods and Results - We used optical mapping techniques to study Langendorff-perfused rabbit hearts. In protocol IA (n=10), D600 at increasing concentrations was infused during ventricular fibrillation (VF). With concentration increased to 0.5 mg/L, fast VF (dominant frequency, 19.1 ± 1.8 Hz) was consistently converted to ventricular tachycardia (VT). However, increasing D600 further to 2.5 or 5.0 mg/L converted VT to slow VF (11.9±2.3 Hz, P=0.0011). In an additional 4 hearts (protocol IB), tetrodotoxin converted a preexisting VT to slow VF (11.0±1.4 Hz). Optical maps show wandering wavelets in fast VF, organized reentry in VT, and spatiotemporal periodicity in slow VF. In protocol II, we determined APD and conduction time-1 (CT-1) restitutions during D600 infusion. CT-1 was used as an estimate of excitability. At 0.1 mg/L, APD and CT-1 restitutions were steep and flat, respectively. APD restitution became flattened when D600 increased to 0.5 mg/L, converting fast VF to VT. Further increasing D600 to 2.5 or 5.0 mg/L steepened CT-1 restitution and widened the range of S1 pacing cycle lengths over which CT-1 decreased, converting VT to slow VF. Conclusions - Two types of VF exist in isolated rabbit hearts. Fast (type I) VF is associated with a steep APD restitution, a flat CT-1 restitution, and wandering wavelets. Slow (type II) VF is associated with a flat APD restitution, a steep CT-1 restitution, and spatiotemporal periodicity. Both excitability and APD restitution are important in VF maintenance.",
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AU - Weiss, James N.

AU - Ting, Chih Tai

AU - Chen, Peng-Sheng

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N2 - Background - The combined effects of excitability and action potential duration (APD) restitution on wavefront dynamics remain unclear. Methods and Results - We used optical mapping techniques to study Langendorff-perfused rabbit hearts. In protocol IA (n=10), D600 at increasing concentrations was infused during ventricular fibrillation (VF). With concentration increased to 0.5 mg/L, fast VF (dominant frequency, 19.1 ± 1.8 Hz) was consistently converted to ventricular tachycardia (VT). However, increasing D600 further to 2.5 or 5.0 mg/L converted VT to slow VF (11.9±2.3 Hz, P=0.0011). In an additional 4 hearts (protocol IB), tetrodotoxin converted a preexisting VT to slow VF (11.0±1.4 Hz). Optical maps show wandering wavelets in fast VF, organized reentry in VT, and spatiotemporal periodicity in slow VF. In protocol II, we determined APD and conduction time-1 (CT-1) restitutions during D600 infusion. CT-1 was used as an estimate of excitability. At 0.1 mg/L, APD and CT-1 restitutions were steep and flat, respectively. APD restitution became flattened when D600 increased to 0.5 mg/L, converting fast VF to VT. Further increasing D600 to 2.5 or 5.0 mg/L steepened CT-1 restitution and widened the range of S1 pacing cycle lengths over which CT-1 decreased, converting VT to slow VF. Conclusions - Two types of VF exist in isolated rabbit hearts. Fast (type I) VF is associated with a steep APD restitution, a flat CT-1 restitution, and wandering wavelets. Slow (type II) VF is associated with a flat APD restitution, a steep CT-1 restitution, and spatiotemporal periodicity. Both excitability and APD restitution are important in VF maintenance.

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