Type XI collagen-degrading activity in human osteoarthritic cartilage

Lucino P. Yu, Gerald N. Smith, Kenneth D. Brandt, William Capello

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Homogenates of 6 samples of human osteoarthritic cartilage were shown to degrade exogenous type XI collagen. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the cleavage products generated by each homogenate were similar, and they were identical to those obtained by cleavage of the substrate with purified gelatinase. Enzyme activity, which was inhibited by EDTA, was greater in extracts of fibrillated osteoarthritic cartilage than in extracts of grossly normal cartilage from the same joint or in extracts of cartilage from joints with osteonecrosis. Activation with APMA enhanced digestion, but breakdown was apparent in extracts of fibrillated osteoarthritic cartilage even without APMA. Enzymatic degradation of type XI collagen could play a significant role in the turnover of articular cartilage in health and disease states.

Original languageEnglish
Pages (from-to)1626-1633
Number of pages8
JournalArthritis and Rheumatism
Volume33
Issue number11
StatePublished - Nov 1990

Fingerprint

Collagen Type XI
Human Activities
Cartilage
Joints
Gelatinases
Osteonecrosis
Articular Cartilage
Edetic Acid
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Digestion
Health
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Type XI collagen-degrading activity in human osteoarthritic cartilage. / Yu, Lucino P.; Smith, Gerald N.; Brandt, Kenneth D.; Capello, William.

In: Arthritis and Rheumatism, Vol. 33, No. 11, 11.1990, p. 1626-1633.

Research output: Contribution to journalArticle

Yu, LP, Smith, GN, Brandt, KD & Capello, W 1990, 'Type XI collagen-degrading activity in human osteoarthritic cartilage', Arthritis and Rheumatism, vol. 33, no. 11, pp. 1626-1633.
Yu, Lucino P. ; Smith, Gerald N. ; Brandt, Kenneth D. ; Capello, William. / Type XI collagen-degrading activity in human osteoarthritic cartilage. In: Arthritis and Rheumatism. 1990 ; Vol. 33, No. 11. pp. 1626-1633.
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