Typing of Histoplasma capsulatum isolates based on nucleotide sequence variation in the internal transcribed spacer regions of rRNA genes

Bingdong Jiang, Marilyn S. Bartlett, Stephen D. Allen, James W. Smith, L. Joseph Wheat, Patricia A. Connolly, Chao-Hung Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The nucleotide sequences of internal transcribed spacer (ITS) regions of rRNA genes of 24 isolates of Histoplasma capsulatum were examined. The results indicate that the sequences of ITS regions in different isolates are not identical. Sequence variations were found at 20 positions in the 496 bp that were sequenced. Ten different sequence patterns, designated types A through H, were observed when the sequences from the 24 isolates were aligned. Twelve isolates from Indianapolis were classified into four different types. Two isolates from New York belonged to type G. Three isolates from different cities were type F. The remaining six isolates were of different types.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalJournal of Clinical Microbiology
Volume38
Issue number1
StatePublished - 2000

Fingerprint

Histoplasma
rRNA Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Typing of Histoplasma capsulatum isolates based on nucleotide sequence variation in the internal transcribed spacer regions of rRNA genes. / Jiang, Bingdong; Bartlett, Marilyn S.; Allen, Stephen D.; Smith, James W.; Wheat, L. Joseph; Connolly, Patricia A.; Lee, Chao-Hung.

In: Journal of Clinical Microbiology, Vol. 38, No. 1, 2000, p. 241-245.

Research output: Contribution to journalArticle

Jiang, Bingdong ; Bartlett, Marilyn S. ; Allen, Stephen D. ; Smith, James W. ; Wheat, L. Joseph ; Connolly, Patricia A. ; Lee, Chao-Hung. / Typing of Histoplasma capsulatum isolates based on nucleotide sequence variation in the internal transcribed spacer regions of rRNA genes. In: Journal of Clinical Microbiology. 2000 ; Vol. 38, No. 1. pp. 241-245.
@article{b1a425ff308a49da814c521a06a3eecc,
title = "Typing of Histoplasma capsulatum isolates based on nucleotide sequence variation in the internal transcribed spacer regions of rRNA genes",
abstract = "The nucleotide sequences of internal transcribed spacer (ITS) regions of rRNA genes of 24 isolates of Histoplasma capsulatum were examined. The results indicate that the sequences of ITS regions in different isolates are not identical. Sequence variations were found at 20 positions in the 496 bp that were sequenced. Ten different sequence patterns, designated types A through H, were observed when the sequences from the 24 isolates were aligned. Twelve isolates from Indianapolis were classified into four different types. Two isolates from New York belonged to type G. Three isolates from different cities were type F. The remaining six isolates were of different types.",
author = "Bingdong Jiang and Bartlett, {Marilyn S.} and Allen, {Stephen D.} and Smith, {James W.} and Wheat, {L. Joseph} and Connolly, {Patricia A.} and Chao-Hung Lee",
year = "2000",
language = "English",
volume = "38",
pages = "241--245",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Typing of Histoplasma capsulatum isolates based on nucleotide sequence variation in the internal transcribed spacer regions of rRNA genes

AU - Jiang, Bingdong

AU - Bartlett, Marilyn S.

AU - Allen, Stephen D.

AU - Smith, James W.

AU - Wheat, L. Joseph

AU - Connolly, Patricia A.

AU - Lee, Chao-Hung

PY - 2000

Y1 - 2000

N2 - The nucleotide sequences of internal transcribed spacer (ITS) regions of rRNA genes of 24 isolates of Histoplasma capsulatum were examined. The results indicate that the sequences of ITS regions in different isolates are not identical. Sequence variations were found at 20 positions in the 496 bp that were sequenced. Ten different sequence patterns, designated types A through H, were observed when the sequences from the 24 isolates were aligned. Twelve isolates from Indianapolis were classified into four different types. Two isolates from New York belonged to type G. Three isolates from different cities were type F. The remaining six isolates were of different types.

AB - The nucleotide sequences of internal transcribed spacer (ITS) regions of rRNA genes of 24 isolates of Histoplasma capsulatum were examined. The results indicate that the sequences of ITS regions in different isolates are not identical. Sequence variations were found at 20 positions in the 496 bp that were sequenced. Ten different sequence patterns, designated types A through H, were observed when the sequences from the 24 isolates were aligned. Twelve isolates from Indianapolis were classified into four different types. Two isolates from New York belonged to type G. Three isolates from different cities were type F. The remaining six isolates were of different types.

UR - http://www.scopus.com/inward/record.url?scp=0033988130&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033988130&partnerID=8YFLogxK

M3 - Article

VL - 38

SP - 241

EP - 245

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -