Ubiquitin ligase activity of TFIIH and the transcriptional response to DNA damage

Yuichiro Takagi, Claudio A. Masuda, Wei Hau Chang, Hirofumi Komori, Dong Wang, Tony Hunter, Claudio A P Joazeiro, Roger D. Kornberg

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Core transcription factor (TF) IIH purified from yeast possesses an E3 ubiquitin (Ub) ligase activity, which resides, at least in part, in a RING finger (RNF) domain of the Ssl1 subunit. Yeast strains mutated in the Ssl1 RNF domain are sensitive to ultraviolet (UV) light and to methyl methanesulfonate (MMS). This increased sensitivity to DNA-damaging agents does not reflect a deficiency in nucleotide excision repair. Rather, it correlates with reduced transcriptional induction of genes involved in DNA repair, suggesting that the E3 Ub ligase activity of TFIIH mediates the transcriptional response to DNA damage.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalMolecular Cell
Volume18
Issue number2
DOIs
StatePublished - Apr 15 2005
Externally publishedYes

Fingerprint

RING Finger Domains
Ubiquitin-Protein Ligases
Ligases
Ubiquitin
DNA Repair
DNA Damage
Transcription Factor TFIIH
Yeasts
Methyl Methanesulfonate
Ultraviolet Rays
DNA
Genes

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Takagi, Y., Masuda, C. A., Chang, W. H., Komori, H., Wang, D., Hunter, T., ... Kornberg, R. D. (2005). Ubiquitin ligase activity of TFIIH and the transcriptional response to DNA damage. Molecular Cell, 18(2), 237-243. https://doi.org/10.1016/j.molcel.2005.03.007

Ubiquitin ligase activity of TFIIH and the transcriptional response to DNA damage. / Takagi, Yuichiro; Masuda, Claudio A.; Chang, Wei Hau; Komori, Hirofumi; Wang, Dong; Hunter, Tony; Joazeiro, Claudio A P; Kornberg, Roger D.

In: Molecular Cell, Vol. 18, No. 2, 15.04.2005, p. 237-243.

Research output: Contribution to journalArticle

Takagi, Y, Masuda, CA, Chang, WH, Komori, H, Wang, D, Hunter, T, Joazeiro, CAP & Kornberg, RD 2005, 'Ubiquitin ligase activity of TFIIH and the transcriptional response to DNA damage', Molecular Cell, vol. 18, no. 2, pp. 237-243. https://doi.org/10.1016/j.molcel.2005.03.007
Takagi, Yuichiro ; Masuda, Claudio A. ; Chang, Wei Hau ; Komori, Hirofumi ; Wang, Dong ; Hunter, Tony ; Joazeiro, Claudio A P ; Kornberg, Roger D. / Ubiquitin ligase activity of TFIIH and the transcriptional response to DNA damage. In: Molecular Cell. 2005 ; Vol. 18, No. 2. pp. 237-243.
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