Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation

Mondira Kundu, Tullia Lindsten, Chia Ying Yang, Junmin Wu, Fangping Zhao, Ji Zhang, Mary A. Selak, Paul A. Ney, Craig B. Thompson

Research output: Contribution to journalArticle

332 Citations (Scopus)

Abstract

Production of a red blood cell's hemoglobin depends on mitochondrial heme synthesis. However, mature red blood cells are devoid of mitochondria and rely on glycolysis for ATP production. The molecular basis for the selective elimination of mitochondria from mature red blood cells remains controversial. Recent evidence suggests that clearance of both mitochondria and ribosomes, which occurs in reticulocytes following nuclear extrusion, depends on autophagy. Here, we demonstrate that Ulk1, a serine threonine kinase with homology to yeast atg1p, is a critical regulator of mitochondrial and ribosomal clearance during the final stages of erythroid maturation. However, in contrast to the core autophagy genes such as atg5 and atg7, expression of ulk1 is not essential for induction of macroautophagy in response to nutrient deprivation or for survival of newborn mice. Together, these data suggest that the ATG1 homologue, Ulk1, is a component of the selective autophagy machinery that leads to the elimination of organelles in erythroid cells rather that an essential mechanistic component of autophagy.

Original languageEnglish (US)
Pages (from-to)1493-1502
Number of pages10
JournalBlood
Volume112
Issue number4
DOIs
StatePublished - Aug 15 2008
Externally publishedYes

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Mitochondria
Reticulocytes
Autophagy
Ribosomes
Blood
Cells
Erythrocytes
Protein-Serine-Threonine Kinases
Heme
Yeast
Nutrients
Machinery
Extrusion
Hemoglobins
Genes
Adenosine Triphosphate
Erythroid Cells
Glycolysis
Organelles
Yeasts

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation. / Kundu, Mondira; Lindsten, Tullia; Yang, Chia Ying; Wu, Junmin; Zhao, Fangping; Zhang, Ji; Selak, Mary A.; Ney, Paul A.; Thompson, Craig B.

In: Blood, Vol. 112, No. 4, 15.08.2008, p. 1493-1502.

Research output: Contribution to journalArticle

Kundu, M, Lindsten, T, Yang, CY, Wu, J, Zhao, F, Zhang, J, Selak, MA, Ney, PA & Thompson, CB 2008, 'Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation', Blood, vol. 112, no. 4, pp. 1493-1502. https://doi.org/10.1182/blood-2008-02-137398
Kundu, Mondira ; Lindsten, Tullia ; Yang, Chia Ying ; Wu, Junmin ; Zhao, Fangping ; Zhang, Ji ; Selak, Mary A. ; Ney, Paul A. ; Thompson, Craig B. / Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation. In: Blood. 2008 ; Vol. 112, No. 4. pp. 1493-1502.
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AU - Selak, Mary A.

AU - Ney, Paul A.

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