Ultrasound-assisted non-viral gene transfer to the salivary glands

M. J. Passineau, L. Zourelias, L. MacHen, Paul Edwards, R. L. Benza

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We report a non-viral gene transfer method using ultrasound induced microbubble destruction to allow the uptake of plasmid gene transfer vectors to the cells of the mouse salivary gland. The Luciferase (Luc) reporter gene, driven by a cytomegalovirus (CMV) promoter, was delivered unilaterally to the submandibular salivary gland via retroductal cannulation and Luc expression was monitored with in vivo imaging. The CMV-Luc plasmid was delivered to the salivary gland in a carrier solution containing microbubbles composed of lipid-encased perfluoropropane gas, with two different concentrations of microbubbles used (100 and 15% volume/volume). An Adenoviral (Ad) vector using an identical CMV-Luc expression cassette was used as a positive control at two different dosages. Whereas ultrasound-assisted gene transfer (UAGT) with 100% microbubbles was weak and rapidly extinguished, UAGT with the 15% microbubble solution was robust and stable for 28 days. UAGT seems to be a practicable and promising method for non-viral gene delivery to the salivary glands.

Original languageEnglish (US)
Pages (from-to)1318-1324
Number of pages7
JournalGene Therapy
Volume17
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

Fingerprint

Microbubbles
Salivary Glands
Luciferases
Cytomegalovirus
Genes
perflutren
Plasmids
Submandibular Gland
Reporter Genes
Catheterization
Gases
Lipids

Keywords

  • gene transfer
  • non-viral
  • salivary glands
  • ultrasound

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Passineau, M. J., Zourelias, L., MacHen, L., Edwards, P., & Benza, R. L. (2010). Ultrasound-assisted non-viral gene transfer to the salivary glands. Gene Therapy, 17(11), 1318-1324. https://doi.org/10.1038/gt.2010.86

Ultrasound-assisted non-viral gene transfer to the salivary glands. / Passineau, M. J.; Zourelias, L.; MacHen, L.; Edwards, Paul; Benza, R. L.

In: Gene Therapy, Vol. 17, No. 11, 11.2010, p. 1318-1324.

Research output: Contribution to journalArticle

Passineau, MJ, Zourelias, L, MacHen, L, Edwards, P & Benza, RL 2010, 'Ultrasound-assisted non-viral gene transfer to the salivary glands', Gene Therapy, vol. 17, no. 11, pp. 1318-1324. https://doi.org/10.1038/gt.2010.86
Passineau, M. J. ; Zourelias, L. ; MacHen, L. ; Edwards, Paul ; Benza, R. L. / Ultrasound-assisted non-viral gene transfer to the salivary glands. In: Gene Therapy. 2010 ; Vol. 17, No. 11. pp. 1318-1324.
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