Umbilical cord and placental blood hematopoietic stem cells: collection, cryopreservation, and storage.

J. E. Wagner, Hal Broxmeyer, S. Cooper

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Human umbilical cord and placental blood is a rich source of hematopoietic stem cells that can be used to reconstitute hematopoiesis after intensive myeloablative therapy. Therefore, it has become necessary to develop techniques for (i) collecting large volumes of umbilical cord aseptically, (ii) optimizing the yield of viable cells after cryopreservation and thawing, and (iii) minimizing the risk of maternal T-cell contamination. Our protocol for the collection, cryopreservation, storage, and transport of human umbilical cord and placental blood is described in detail.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalJournal of Hematotherapy
Volume1
Issue number2
StatePublished - Jun 1992

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Cryopreservation
Hematopoietic Stem Cells
Fetal Blood
Umbilical Cord
Hematopoiesis
Mothers
T-Lymphocytes
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Umbilical cord and placental blood hematopoietic stem cells : collection, cryopreservation, and storage. / Wagner, J. E.; Broxmeyer, Hal; Cooper, S.

In: Journal of Hematotherapy, Vol. 1, No. 2, 06.1992, p. 167-173.

Research output: Contribution to journalArticle

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