Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction

Deborah E. Isaksen, Heinz Baumann, Baohua Zhou, Sebastien Nivollet, Andrew G. Farr, Steven D. Levin, Steven F. Ziegler

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Thymic stromal lymphopoietin (TSLP) is a cytokine that facilitates B lymphocyte differentiation and costimulates T cells. Previous studies have demonstrated that a functional TSLP receptor complex is a heterodimer consisting of the TSLP receptor and the IL-7R α-chain. TSLP-mediated signaling is unique among members of the cytokine receptor family in that activation of the transcription factor Stat5 occurs without detectable Janus kinase activation. Using a variety of biological systems we demonstrate here that TSLP-mediated Stat5 activation can be uncoupled from proliferation. We also show that the single tyrosine residue in the cytoplasmic domain of the TSLP receptor is critical for TSLP-mediated proliferation, but is dispensable for Stat5 activation. Our data demonstrate that TSLP-mediated Stat5 activation is insufficient for cell proliferation and identifies residues within the TSLP receptor complex required to mediate these downstream events.

Original languageEnglish (US)
Pages (from-to)3288-3294
Number of pages7
JournalJournal of Immunology
Volume168
Issue number7
StatePublished - Apr 1 2002
Externally publishedYes

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Signal Transduction
Janus Kinases
thymic stromal lymphopoietin
Cytokine Receptors
Tyrosine
B-Lymphocytes
Transcription Factors
Cell Proliferation
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Isaksen, D. E., Baumann, H., Zhou, B., Nivollet, S., Farr, A. G., Levin, S. D., & Ziegler, S. F. (2002). Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction. Journal of Immunology, 168(7), 3288-3294.

Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction. / Isaksen, Deborah E.; Baumann, Heinz; Zhou, Baohua; Nivollet, Sebastien; Farr, Andrew G.; Levin, Steven D.; Ziegler, Steven F.

In: Journal of Immunology, Vol. 168, No. 7, 01.04.2002, p. 3288-3294.

Research output: Contribution to journalArticle

Isaksen, DE, Baumann, H, Zhou, B, Nivollet, S, Farr, AG, Levin, SD & Ziegler, SF 2002, 'Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction', Journal of Immunology, vol. 168, no. 7, pp. 3288-3294.
Isaksen DE, Baumann H, Zhou B, Nivollet S, Farr AG, Levin SD et al. Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction. Journal of Immunology. 2002 Apr 1;168(7):3288-3294.
Isaksen, Deborah E. ; Baumann, Heinz ; Zhou, Baohua ; Nivollet, Sebastien ; Farr, Andrew G. ; Levin, Steven D. ; Ziegler, Steven F. / Uncoupling of proliferation and Stat5 activation in thymic stromal lymphopoietin-mediated signal transduction. In: Journal of Immunology. 2002 ; Vol. 168, No. 7. pp. 3288-3294.
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