Unique cellular events occurring during the initial interaction of macrophages with matrix-retained or methylated aggregated low density lipoprotein (LDL). Prolonged cell-surface contact during which LDL- cholesteryl ester hydrolysis exceeds LDL protein degradation

Xavier Buton, Zahra Mamdouh, Richik Ghosh, Hong Du, George Kuriakose, Nanda Beatini, Gregory A. Grabowski, Frederick R. Maxfield, Ira Tabast

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A critical event in atherogenesis is the interaction of arterial wall macrophages with subendothelial lipoproteins. Although most studies have investigated this interaction by incubating cultured macrophages with moo nomeric lipoproteins dissolved in media, arterial wall macrophages encounter lipoproteins that are mostly bound to subendothelial extracellular matrix, and these lipoproteins are often aggregated or fused. Herein, we utilize a specialized cell-culture system to study the initial interaction of macrophages with aggregated low density lipoprotein (LDL) bound to extracellular matrix. The aggregated LDL remains extracellular for a relatively prolonged period of time and becomes lodged in invaginations in the surface of the macrophages. As expected, the degradation of the protein moiety of the LDL was very slow. Remarkably, however, hydrolysis of the cholesteryl ester (CE) moiety of the LDL was 3-7-fold higher than that of the protein moiety, in stark contrast to the situation with receptor-mediated endocytosis of acetyl-LDL. Similar results were obtained using another experimental system in which the degradation of aggregated LDL protein was delayed by LDL methylation rather than by retention on matrix. Additional experiments indicated the following properties of this interaction: (a) LDL- CE hydrolysis is catalyzed by lysosomal acid lipase; (b) neither scavenger receptors nor the LDL receptor appear necessary for the excess LDL-CE hydrolysis; and (c) LDL-CE hydrolysis in this system is resistant to cellular potassium depletion, which further distinguishes this process from receptor- mediated endocytosis. In summary, experimental systems specifically designed to mimic the in vivo interaction of arterial wall macrophages with subendothelial lipoproteins have demonstrated an initial period of prolonged cell-surface contact in which CE hydrolysis exceeds protein degradation.

Original languageEnglish (US)
Pages (from-to)32112-32121
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number45
DOIs
StatePublished - Nov 5 1999
Externally publishedYes

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Cholesterol Esters
Macrophages
LDL Lipoproteins
Proteolysis
Hydrolysis
Degradation
Lipoproteins
Proteins
Endocytosis
Extracellular Matrix
Tunica Media
Sterol Esterase
Scavenger Receptors
VLDL Lipoproteins
LDL Receptors
Methylation
Cell culture
Atherosclerosis
Potassium
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Unique cellular events occurring during the initial interaction of macrophages with matrix-retained or methylated aggregated low density lipoprotein (LDL). Prolonged cell-surface contact during which LDL- cholesteryl ester hydrolysis exceeds LDL protein degradation. / Buton, Xavier; Mamdouh, Zahra; Ghosh, Richik; Du, Hong; Kuriakose, George; Beatini, Nanda; Grabowski, Gregory A.; Maxfield, Frederick R.; Tabast, Ira.

In: Journal of Biological Chemistry, Vol. 274, No. 45, 05.11.1999, p. 32112-32121.

Research output: Contribution to journalArticle

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AU - Buton, Xavier

AU - Mamdouh, Zahra

AU - Ghosh, Richik

AU - Du, Hong

AU - Kuriakose, George

AU - Beatini, Nanda

AU - Grabowski, Gregory A.

AU - Maxfield, Frederick R.

AU - Tabast, Ira

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N2 - A critical event in atherogenesis is the interaction of arterial wall macrophages with subendothelial lipoproteins. Although most studies have investigated this interaction by incubating cultured macrophages with moo nomeric lipoproteins dissolved in media, arterial wall macrophages encounter lipoproteins that are mostly bound to subendothelial extracellular matrix, and these lipoproteins are often aggregated or fused. Herein, we utilize a specialized cell-culture system to study the initial interaction of macrophages with aggregated low density lipoprotein (LDL) bound to extracellular matrix. The aggregated LDL remains extracellular for a relatively prolonged period of time and becomes lodged in invaginations in the surface of the macrophages. As expected, the degradation of the protein moiety of the LDL was very slow. Remarkably, however, hydrolysis of the cholesteryl ester (CE) moiety of the LDL was 3-7-fold higher than that of the protein moiety, in stark contrast to the situation with receptor-mediated endocytosis of acetyl-LDL. Similar results were obtained using another experimental system in which the degradation of aggregated LDL protein was delayed by LDL methylation rather than by retention on matrix. Additional experiments indicated the following properties of this interaction: (a) LDL- CE hydrolysis is catalyzed by lysosomal acid lipase; (b) neither scavenger receptors nor the LDL receptor appear necessary for the excess LDL-CE hydrolysis; and (c) LDL-CE hydrolysis in this system is resistant to cellular potassium depletion, which further distinguishes this process from receptor- mediated endocytosis. In summary, experimental systems specifically designed to mimic the in vivo interaction of arterial wall macrophages with subendothelial lipoproteins have demonstrated an initial period of prolonged cell-surface contact in which CE hydrolysis exceeds protein degradation.

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