Unitary anion currents through phospholemman channel molecules

J. Randall Moorman, Stephen J. Ackerman, Gopal C. Kowdley, M. Pamela Griffin, J. Paul Mounsey, Zhenhui Chen, Steven E. Cala, Jeffery J. O’Brian, Gabor Szabo, Larry R. Jones

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

PHOSPHOLEMMAN (PLM) is a 72-amino-acid peptide with a single transmembrane domain1-3, the expression of which induces chloride currents in Xenopus oocytes4-6. It has remained unknown whether PLM is an ion channel or acts as a channel regulator. Here we show, by measuring unitary anion currents across planar phospholipid bilayers to which immunoaffinity-purified recombinant PLM was added, that it does indeed form ion channels. Excised patches of oocytes expressing PLM had similar currents. Of the ions tested, the sulphonic amino acid taurine was the most permeant, and expression of PLM increased fluxes of radiolabelled taurine in oocytes. Phospholemman is the smallest protein in cell membranes known to form an ion channel and the taurine selectivity suggests that it is involved in cell volume regulation.

Original languageEnglish (US)
Pages (from-to)737-740
Number of pages4
JournalNature
Volume377
Issue number6551
DOIs
StatePublished - Oct 26 1995

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Taurine
Ion Channels
Anions
Oocytes
Amino Acids
Sulfonic Acids
Xenopus
Cell Size
Chlorides
Phospholipids
Membrane Proteins
Ions
Peptides
phospholemman

ASJC Scopus subject areas

  • General

Cite this

Moorman, J. R., Ackerman, S. J., Kowdley, G. C., Griffin, M. P., Mounsey, J. P., Chen, Z., ... Jones, L. R. (1995). Unitary anion currents through phospholemman channel molecules. Nature, 377(6551), 737-740. https://doi.org/10.1038/377737a0

Unitary anion currents through phospholemman channel molecules. / Moorman, J. Randall; Ackerman, Stephen J.; Kowdley, Gopal C.; Griffin, M. Pamela; Mounsey, J. Paul; Chen, Zhenhui; Cala, Steven E.; O’Brian, Jeffery J.; Szabo, Gabor; Jones, Larry R.

In: Nature, Vol. 377, No. 6551, 26.10.1995, p. 737-740.

Research output: Contribution to journalArticle

Moorman, JR, Ackerman, SJ, Kowdley, GC, Griffin, MP, Mounsey, JP, Chen, Z, Cala, SE, O’Brian, JJ, Szabo, G & Jones, LR 1995, 'Unitary anion currents through phospholemman channel molecules', Nature, vol. 377, no. 6551, pp. 737-740. https://doi.org/10.1038/377737a0
Moorman JR, Ackerman SJ, Kowdley GC, Griffin MP, Mounsey JP, Chen Z et al. Unitary anion currents through phospholemman channel molecules. Nature. 1995 Oct 26;377(6551):737-740. https://doi.org/10.1038/377737a0
Moorman, J. Randall ; Ackerman, Stephen J. ; Kowdley, Gopal C. ; Griffin, M. Pamela ; Mounsey, J. Paul ; Chen, Zhenhui ; Cala, Steven E. ; O’Brian, Jeffery J. ; Szabo, Gabor ; Jones, Larry R. / Unitary anion currents through phospholemman channel molecules. In: Nature. 1995 ; Vol. 377, No. 6551. pp. 737-740.
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