Unraveling the complexities of DNA-dependent protein kinase autophosphorylation

Jessica A. Neal, Seiji Sugiman-Marangos, Pamela VanderVere-Carozza, Mike Wagner, John Turchi, Susan P. Lees-Miller, Murray S. Junop, Katheryn Meek

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

DNA-dependent protein kinase (DNA-PK) orchestrates DNA repair by regulating access to breaks through autophosphorylations within two clusters of sites (ABCDE and PQR). Blocking ABCDE phosphorylation (by alanine mutation) imparts a dominant negative effect, rendering cells hypersensitive to agents that cause DNA double-strand breaks. Here, a mutational approach is used to address the mechanistic basis of this dominant negative effect. Blocking ABCDE phosphorylation hypersensitizes cells to most types of DNA damage (base damage, cross-links, breaks, and damage induced by replication stress), suggesting that DNA-PK binds DNA ends that result from many DNA lesions and that blocking ABCDE phosphorylation sequesters these DNA ends from other repair pathways. This dominant negative effect requires DNA-PK's catalytic activity, as well as phosphorylation of multiple (non-ABCDE) DNA-PK catalytic subunit (DNA-PKcs) sites. PSIPRED analysis indicates that the ABCDE sites are located in the only contiguous extended region of this huge protein that is predicted to be disordered, suggesting a regulatory role(s) and perhaps explaining the large impact ABCDE phosphorylation has on the enzyme's function. Moreover, additional sites in this disordered region contribute to the ABCDE cluster. These data, coupled with recent structural data, suggest a model whereby early phosphorylations promote initiation of nonhomologous end joining (NHEJ), whereas ABCDE phosphorylations, potentially located in a "hinge" region between the two domains, lead to regulated conformational changes that initially promote NHEJ and eventually disengage NHEJ

Original languageEnglish
Pages (from-to)2162-2175
Number of pages14
JournalMolecular and Cellular Biology
Volume34
Issue number12
DOIs
StatePublished - 2014

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DNA-Activated Protein Kinase
Phosphorylation
DNA
Catalytic Domain
Catalytic DNA
Double-Stranded DNA Breaks
DNA Repair
Alanine
DNA Damage
Mutation
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Neal, J. A., Sugiman-Marangos, S., VanderVere-Carozza, P., Wagner, M., Turchi, J., Lees-Miller, S. P., ... Meek, K. (2014). Unraveling the complexities of DNA-dependent protein kinase autophosphorylation. Molecular and Cellular Biology, 34(12), 2162-2175. https://doi.org/10.1128/MCB.01554-13

Unraveling the complexities of DNA-dependent protein kinase autophosphorylation. / Neal, Jessica A.; Sugiman-Marangos, Seiji; VanderVere-Carozza, Pamela; Wagner, Mike; Turchi, John; Lees-Miller, Susan P.; Junop, Murray S.; Meek, Katheryn.

In: Molecular and Cellular Biology, Vol. 34, No. 12, 2014, p. 2162-2175.

Research output: Contribution to journalArticle

Neal, JA, Sugiman-Marangos, S, VanderVere-Carozza, P, Wagner, M, Turchi, J, Lees-Miller, SP, Junop, MS & Meek, K 2014, 'Unraveling the complexities of DNA-dependent protein kinase autophosphorylation', Molecular and Cellular Biology, vol. 34, no. 12, pp. 2162-2175. https://doi.org/10.1128/MCB.01554-13
Neal JA, Sugiman-Marangos S, VanderVere-Carozza P, Wagner M, Turchi J, Lees-Miller SP et al. Unraveling the complexities of DNA-dependent protein kinase autophosphorylation. Molecular and Cellular Biology. 2014;34(12):2162-2175. https://doi.org/10.1128/MCB.01554-13
Neal, Jessica A. ; Sugiman-Marangos, Seiji ; VanderVere-Carozza, Pamela ; Wagner, Mike ; Turchi, John ; Lees-Miller, Susan P. ; Junop, Murray S. ; Meek, Katheryn. / Unraveling the complexities of DNA-dependent protein kinase autophosphorylation. In: Molecular and Cellular Biology. 2014 ; Vol. 34, No. 12. pp. 2162-2175.
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