Unusual Escherichia coli PBP 3 insertion sequence identified from a collection of carbapenem-resistant Enterobacteriaceae tested in vitro with a combination of ceftazidime-, ceftaroline-, or aztreonam-avibactam

Yunliang Zhang, Ankita Kashikar, C. Adam Brown, Gerald Denys, Karen Bush

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8 Citations (Scopus)

Abstract

Carbapenemase-producing Enterobacteriaceae isolates (n = 110) from health care centers in central Indiana (from 2010 to 2013) were tested for susceptibility to combinations of avibactam (4 μg/ml) with ceftazidime, ceftaroline, or aztreonam. MIC 50 /MIC 90 values were 1/2 μg/ml (ceftazidime-avibactam), 0.5/2 μg/ml (ceftaroline-avibactam), and 0.25/0.5 μg/ml (aztreonam-avibactam.) A β-lactam MIC of 8 μg/ml was reported for the three combinations against one Escherichia coli isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication.

Original languageEnglish (US)
Article numbere00389
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number8
DOIs
StatePublished - Aug 1 2017

Fingerprint

Penicillin-Binding Proteins
Aztreonam
Carbapenems
Ceftazidime
Insertional Mutagenesis
Escherichia coli Proteins
Enterobacteriaceae
Lactams
Gene Duplication
Escherichia coli
Delivery of Health Care
avibactam
In Vitro Techniques
T 91825

Keywords

  • Avibactam
  • Aztreonam
  • Carbapenem-resistant
  • Carbapenemase
  • Ceftaroline
  • Ceftazidime
  • CRE
  • PBP 3
  • Resistance

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

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title = "Unusual Escherichia coli PBP 3 insertion sequence identified from a collection of carbapenem-resistant Enterobacteriaceae tested in vitro with a combination of ceftazidime-, ceftaroline-, or aztreonam-avibactam",
abstract = "Carbapenemase-producing Enterobacteriaceae isolates (n = 110) from health care centers in central Indiana (from 2010 to 2013) were tested for susceptibility to combinations of avibactam (4 μg/ml) with ceftazidime, ceftaroline, or aztreonam. MIC 50 /MIC 90 values were 1/2 μg/ml (ceftazidime-avibactam), 0.5/2 μg/ml (ceftaroline-avibactam), and 0.25/0.5 μg/ml (aztreonam-avibactam.) A β-lactam MIC of 8 μg/ml was reported for the three combinations against one Escherichia coli isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication.",
keywords = "Avibactam, Aztreonam, Carbapenem-resistant, Carbapenemase, Ceftaroline, Ceftazidime, CRE, PBP 3, Resistance",
author = "Yunliang Zhang and Ankita Kashikar and Brown, {C. Adam} and Gerald Denys and Karen Bush",
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AU - Zhang, Yunliang

AU - Kashikar, Ankita

AU - Brown, C. Adam

AU - Denys, Gerald

AU - Bush, Karen

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AB - Carbapenemase-producing Enterobacteriaceae isolates (n = 110) from health care centers in central Indiana (from 2010 to 2013) were tested for susceptibility to combinations of avibactam (4 μg/ml) with ceftazidime, ceftaroline, or aztreonam. MIC 50 /MIC 90 values were 1/2 μg/ml (ceftazidime-avibactam), 0.5/2 μg/ml (ceftaroline-avibactam), and 0.25/0.5 μg/ml (aztreonam-avibactam.) A β-lactam MIC of 8 μg/ml was reported for the three combinations against one Escherichia coli isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication.

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KW - Resistance

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