Upregulation of both PDGF-BB and PDGF-BB receptor in human bladder fibroblasts in response to physiologic hydrostatic pressure

Cem Akbal, Sang Don Lee, Chaeyong Jung, Richard Rink, Martin Kaefer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: Bladder outlet obstruction can lead to the deposition of extracellular matrix and a resultant decrease in bladder wall compliance. Platelet-derived growth factor (PDGF) is a potent mitogen for fibroblasts and can increase the deposition of extracellular matrix. We attempt to determine if the expression of PDGF-BB and its receptor are altered in human bladder fibroblasts and bladder smooth muscle cells when exposed to hydrostatic pressures in the physiologic range. Materials and methods: Cultured human bladder fibroblasts and smooth muscle cells were evaluated in vitro by using a novel device that controls for hydrostatic pressure. Cells were exposed to pressures of 20 and 40 cmH2O for up to 72 h. Western blot analyses and RT-PCR were performed to evaluate expression of both PDGF-BB and PDGF-BB receptor. Results: PDGF-BB and its receptor increased up to 22-fold and 8-fold, respectively, when human bladder fibroblasts were exposed to 40 cmH2O sustained hydrostatic pressure, while at 20 cmH2O the effect was minimal until 72 h. mRNA for the PDGF-BB receptor in human bladder fibroblasts increased in comparison to control. Western blot analyses demonstrated that exposure of human bladder smooth muscle cells to a sustained hydrostatic pressure of 20 and 40 cmH2O for up to 72 h did not alter expression of either PDGF-BB or its receptor. Conclusions: Both PDGF-BB and its receptor in human bladder fibroblasts were upregulated in a time- and pressure-dependent manner after as little as 24 h exposure to pressures of ≤40 cmH2O. Our results provide support for a potential role of both PDGF-BB and its receptor in bladder fibrosis secondary to increased intravesical pressure. Newer selective PDGF receptor antagonists may prove beneficial in preventing bladder wall fibrosis in patients with either anatomic or functional bladder outlet obstruction.

Original languageEnglish
Pages (from-to)402-408
Number of pages7
JournalJournal of Pediatric Urology
Volume2
Issue number5
DOIs
StatePublished - Oct 2006

Fingerprint

Platelet-Derived Growth Factor Receptors
Hydrostatic Pressure
Urinary Bladder
Up-Regulation
Fibroblasts
Smooth Muscle Myocytes
Urinary Bladder Neck Obstruction
Pressure
Extracellular Matrix
Fibrosis
Western Blotting
platelet-derived growth factor BB
Platelet-Derived Growth Factor
Mitogens
Compliance

Keywords

  • Bladder fibroblasts
  • PDGF-BB
  • PDGF-BB receptor
  • Pressure

ASJC Scopus subject areas

  • Urology
  • Pediatrics, Perinatology, and Child Health

Cite this

Upregulation of both PDGF-BB and PDGF-BB receptor in human bladder fibroblasts in response to physiologic hydrostatic pressure. / Akbal, Cem; Lee, Sang Don; Jung, Chaeyong; Rink, Richard; Kaefer, Martin.

In: Journal of Pediatric Urology, Vol. 2, No. 5, 10.2006, p. 402-408.

Research output: Contribution to journalArticle

@article{9edb03c5196340e7aa8e8e7a9984a33f,
title = "Upregulation of both PDGF-BB and PDGF-BB receptor in human bladder fibroblasts in response to physiologic hydrostatic pressure",
abstract = "Objective: Bladder outlet obstruction can lead to the deposition of extracellular matrix and a resultant decrease in bladder wall compliance. Platelet-derived growth factor (PDGF) is a potent mitogen for fibroblasts and can increase the deposition of extracellular matrix. We attempt to determine if the expression of PDGF-BB and its receptor are altered in human bladder fibroblasts and bladder smooth muscle cells when exposed to hydrostatic pressures in the physiologic range. Materials and methods: Cultured human bladder fibroblasts and smooth muscle cells were evaluated in vitro by using a novel device that controls for hydrostatic pressure. Cells were exposed to pressures of 20 and 40 cmH2O for up to 72 h. Western blot analyses and RT-PCR were performed to evaluate expression of both PDGF-BB and PDGF-BB receptor. Results: PDGF-BB and its receptor increased up to 22-fold and 8-fold, respectively, when human bladder fibroblasts were exposed to 40 cmH2O sustained hydrostatic pressure, while at 20 cmH2O the effect was minimal until 72 h. mRNA for the PDGF-BB receptor in human bladder fibroblasts increased in comparison to control. Western blot analyses demonstrated that exposure of human bladder smooth muscle cells to a sustained hydrostatic pressure of 20 and 40 cmH2O for up to 72 h did not alter expression of either PDGF-BB or its receptor. Conclusions: Both PDGF-BB and its receptor in human bladder fibroblasts were upregulated in a time- and pressure-dependent manner after as little as 24 h exposure to pressures of ≤40 cmH2O. Our results provide support for a potential role of both PDGF-BB and its receptor in bladder fibrosis secondary to increased intravesical pressure. Newer selective PDGF receptor antagonists may prove beneficial in preventing bladder wall fibrosis in patients with either anatomic or functional bladder outlet obstruction.",
keywords = "Bladder fibroblasts, PDGF-BB, PDGF-BB receptor, Pressure",
author = "Cem Akbal and Lee, {Sang Don} and Chaeyong Jung and Richard Rink and Martin Kaefer",
year = "2006",
month = "10",
doi = "10.1016/j.jpurol.2005.08.004",
language = "English",
volume = "2",
pages = "402--408",
journal = "Journal of Pediatric Urology",
issn = "1477-5131",
publisher = "Elsevier BV",
number = "5",

}

TY - JOUR

T1 - Upregulation of both PDGF-BB and PDGF-BB receptor in human bladder fibroblasts in response to physiologic hydrostatic pressure

AU - Akbal, Cem

AU - Lee, Sang Don

AU - Jung, Chaeyong

AU - Rink, Richard

AU - Kaefer, Martin

PY - 2006/10

Y1 - 2006/10

N2 - Objective: Bladder outlet obstruction can lead to the deposition of extracellular matrix and a resultant decrease in bladder wall compliance. Platelet-derived growth factor (PDGF) is a potent mitogen for fibroblasts and can increase the deposition of extracellular matrix. We attempt to determine if the expression of PDGF-BB and its receptor are altered in human bladder fibroblasts and bladder smooth muscle cells when exposed to hydrostatic pressures in the physiologic range. Materials and methods: Cultured human bladder fibroblasts and smooth muscle cells were evaluated in vitro by using a novel device that controls for hydrostatic pressure. Cells were exposed to pressures of 20 and 40 cmH2O for up to 72 h. Western blot analyses and RT-PCR were performed to evaluate expression of both PDGF-BB and PDGF-BB receptor. Results: PDGF-BB and its receptor increased up to 22-fold and 8-fold, respectively, when human bladder fibroblasts were exposed to 40 cmH2O sustained hydrostatic pressure, while at 20 cmH2O the effect was minimal until 72 h. mRNA for the PDGF-BB receptor in human bladder fibroblasts increased in comparison to control. Western blot analyses demonstrated that exposure of human bladder smooth muscle cells to a sustained hydrostatic pressure of 20 and 40 cmH2O for up to 72 h did not alter expression of either PDGF-BB or its receptor. Conclusions: Both PDGF-BB and its receptor in human bladder fibroblasts were upregulated in a time- and pressure-dependent manner after as little as 24 h exposure to pressures of ≤40 cmH2O. Our results provide support for a potential role of both PDGF-BB and its receptor in bladder fibrosis secondary to increased intravesical pressure. Newer selective PDGF receptor antagonists may prove beneficial in preventing bladder wall fibrosis in patients with either anatomic or functional bladder outlet obstruction.

AB - Objective: Bladder outlet obstruction can lead to the deposition of extracellular matrix and a resultant decrease in bladder wall compliance. Platelet-derived growth factor (PDGF) is a potent mitogen for fibroblasts and can increase the deposition of extracellular matrix. We attempt to determine if the expression of PDGF-BB and its receptor are altered in human bladder fibroblasts and bladder smooth muscle cells when exposed to hydrostatic pressures in the physiologic range. Materials and methods: Cultured human bladder fibroblasts and smooth muscle cells were evaluated in vitro by using a novel device that controls for hydrostatic pressure. Cells were exposed to pressures of 20 and 40 cmH2O for up to 72 h. Western blot analyses and RT-PCR were performed to evaluate expression of both PDGF-BB and PDGF-BB receptor. Results: PDGF-BB and its receptor increased up to 22-fold and 8-fold, respectively, when human bladder fibroblasts were exposed to 40 cmH2O sustained hydrostatic pressure, while at 20 cmH2O the effect was minimal until 72 h. mRNA for the PDGF-BB receptor in human bladder fibroblasts increased in comparison to control. Western blot analyses demonstrated that exposure of human bladder smooth muscle cells to a sustained hydrostatic pressure of 20 and 40 cmH2O for up to 72 h did not alter expression of either PDGF-BB or its receptor. Conclusions: Both PDGF-BB and its receptor in human bladder fibroblasts were upregulated in a time- and pressure-dependent manner after as little as 24 h exposure to pressures of ≤40 cmH2O. Our results provide support for a potential role of both PDGF-BB and its receptor in bladder fibrosis secondary to increased intravesical pressure. Newer selective PDGF receptor antagonists may prove beneficial in preventing bladder wall fibrosis in patients with either anatomic or functional bladder outlet obstruction.

KW - Bladder fibroblasts

KW - PDGF-BB

KW - PDGF-BB receptor

KW - Pressure

UR - http://www.scopus.com/inward/record.url?scp=33750612515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750612515&partnerID=8YFLogxK

U2 - 10.1016/j.jpurol.2005.08.004

DO - 10.1016/j.jpurol.2005.08.004

M3 - Article

C2 - 18947646

AN - SCOPUS:33750612515

VL - 2

SP - 402

EP - 408

JO - Journal of Pediatric Urology

JF - Journal of Pediatric Urology

SN - 1477-5131

IS - 5

ER -