Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats

Yan Ting Wang, Shu Han, Kai Hua Zhang, Yu Jin, Xiao-Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

AIM: To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of heparin-binding growth-associated molecule (HB-GAM). METHODS: In a spinal cord transection model, HB-GAM expression and cellular localization were examined using Northern blot, RT-PCR, immunohistochemistry and immunofluorescence double-labeling methods. RESULTS: HB-GAM mRNA was significantly upregulated in spinal cord tissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3 d, peaked at 7 d, and declined to the baseline level at 28 d post-injury. During its peak expression, HB-GAM was colocalized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinal transection. CONCLUSION: SCI induces HB-GAM expression at both mRNA and protein levels in areas close to the injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process of injury and/or repair following SCI.

Original languageEnglish (US)
Pages (from-to)611-616
Number of pages6
JournalActa Pharmacologica Sinica
Volume25
Issue number5
StatePublished - May 2004
Externally publishedYes

Fingerprint

Spinal Cord Injuries
Rats
Up-Regulation
Wounds and Injuries
Gene expression
Neurons
Spinal Cord
Tissue
Gene Expression
Messenger RNA
pleiotrophin
Oligodendroglia
Neuroglia
Astrocytes
Northern Blotting
Labeling
Fluorescent Antibody Technique
Repair
Immunohistochemistry
Polymerase Chain Reaction

Keywords

  • Astrocytes
  • Heparin-binding growth-associated molecule
  • Immunohistochemistry
  • Neurons
  • Reverse transcriptase polymerase chain reaction
  • Spinal cord injuries

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology

Cite this

Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats. / Wang, Yan Ting; Han, Shu; Zhang, Kai Hua; Jin, Yu; Xu, Xiao-Ming; Lu, Pei Hua.

In: Acta Pharmacologica Sinica, Vol. 25, No. 5, 05.2004, p. 611-616.

Research output: Contribution to journalArticle

Wang, Yan Ting ; Han, Shu ; Zhang, Kai Hua ; Jin, Yu ; Xu, Xiao-Ming ; Lu, Pei Hua. / Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats. In: Acta Pharmacologica Sinica. 2004 ; Vol. 25, No. 5. pp. 611-616.
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