Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats

Yan Ting Wang, Shu Han, Kai Hua Zhang, Yu Jin, Xiao Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

AIM: To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of heparin-binding growth-associated molecule (HB-GAM). METHODS: In a spinal cord transection model, HB-GAM expression and cellular localization were examined using Northern blot, RT-PCR, immunohistochemistry and immunofluorescence double-labeling methods. RESULTS: HB-GAM mRNA was significantly upregulated in spinal cord tissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3 d, peaked at 7 d, and declined to the baseline level at 28 d post-injury. During its peak expression, HB-GAM was colocalized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinal transection. CONCLUSION: SCI induces HB-GAM expression at both mRNA and protein levels in areas close to the injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process of injury and/or repair following SCI.

Original languageEnglish (US)
Pages (from-to)611-616
Number of pages6
JournalActa Pharmacologica Sinica
Volume25
Issue number5
StatePublished - May 1 2004
Externally publishedYes

Keywords

  • Astrocytes
  • Heparin-binding growth-associated molecule
  • Immunohistochemistry
  • Neurons
  • Reverse transcriptase polymerase chain reaction
  • Spinal cord injuries

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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