Upregulation of type I interleukin-1 receptor after traumatic spinal cord injury in adult rats

Xiao Fei Wang, Li Dong Huang, Pan Pan Yu, Jian Guo Hu, Lan Yin, Li Wang, Xiao Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Post-traumatic inflammation response has been implicated in secondary injury mechanisms after spinal cord injury (SCI). Interleukin-1 (IL-1) is a key inflammatory mediator that is increasingly expressed after SCI. The action of IL-1 is mediated through its functional receptor, type I interleukin-1 receptor (IL-1RI). However, whether this receptor is expressed after SCI remains to be elucidated. In the present study, the temporospatial expression of IL-1RI was detected in rats that received a moderate contusive SCI (a 10 g rod dropped at a height of 12.5 mm) at the ninth to tenth thoracic vertebral level using a widely used New York University impact device. Our study demonstrated that IL-1RI was slightly increased at 4 h post-injury compared to the normal or sham-operated controls, reached the peak at 8 h at mRNA level (4.44-fold, P <0.01) and 1 d at protein level (2.62-fold, P <0.01). IL-1RI remained at its elevated levels for a relatively long duration (4 h-7 days). Spatially, IL-1RI was observed throughout the entire length of a 10 mm-long cord segment containing the injury epicenter. Colocalization of IL-1RI was found in neurons, oligodendrocytes, astrocytes, and activated microglia. Our results suggest that the elevated expression of IL-1RI after SCI may contribute to posttraumatic inflammation responses of IL-1.

Original languageEnglish (US)
Pages (from-to)220-228
Number of pages9
JournalActa Neuropathologica
Volume111
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Keywords

  • IL-1
  • IL-1 receptor
  • Inflammation
  • Spinalcord injury rat

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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