Use of a cell line to investigate olfactory ensheathing cell-enhanced axonal regeneration

Tracey A. DeLucia, James J. Conners, Todd J. Brown, Colleen M. Cronin, Talat Khan, Kathryn J. Jones

Research output: Contribution to journalReview article

33 Scopus citations

Abstract

Olfactory ensheathing cells (OECs), a unique type of macrogila required for normal olfactory axonal regeneration throughout the lifetime of an individual, have been shown to have regeneration-enhancing properties when used to treat various neuronal injuries. Availability of OECs is a hurdle facing future clinical use of the cells for spinal cord injury (SCI) therapy. The number of OECs that can realistically be harvested from each animal is limited, and ensuring a pure cell population is difficult. We have begun to characterize a nonsyngeneic strain of OECS, i.e., from a homogenous OEC clonal cell line (nOECs). The purpose of this study was to determine whether nOECs have the same properties and provide the same functional recovery after SCI, as primary cultures of OECS. The results indicate that nOECs survive in vivo, produce growth-promoting proteins, and possess regeneration-promoting capabilities. Spinal cord injured rats that were treated with nOECs performed significantly better on functional tests than injured control animals beginning at 5 weeks after operation. In summary, evidence of nOEC regeneration-promoting capabilities suggests that this cell line can be used as potential therapy in SCI research.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalAnatomical Record - Part B New Anatomist
Volume271
Issue number1
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Keywords

  • Neuroscience
  • Neurotrophic factors
  • OEC
  • Olfactory glia
  • Regeneration
  • Spinal cord

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Fingerprint Dive into the research topics of 'Use of a cell line to investigate olfactory ensheathing cell-enhanced axonal regeneration'. Together they form a unique fingerprint.

  • Cite this