Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precurosors

Anil K. Tyagi, Hiremagular N. Jayaram, Spurgeon Anandaraj, Benjamin Taylor, David A. Cooney

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A simple and sensitive method has been developed to separate nucleic acid bases, nucleosides, nucleotides and their precursors by automated chromatography using the amino acid analyzer with lithium citrate buffers. The method is sensitive to a concentration of 5 nmol, linear in the range of 5-100 nmol, and resolves almost all the bases, nucleosides, nucleotides and their precursors of physiologic importance.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalJournal of Biochemical and Biophysical Methods
Volume1
Issue number4
DOIs
StatePublished - Aug 1979

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Keywords

  • chromatography
  • lithium chloride
  • lithium citrate
  • nucleic acid bases
  • nucleosides
  • nucleotides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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