Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precurosors

Anil K. Tyagi, Hiremagular N. Jayaram, Spurgeon Anandaraj, Benjamin Taylor, David A. Cooney

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A simple and sensitive method has been developed to separate nucleic acid bases, nucleosides, nucleotides and their precursors by automated chromatography using the amino acid analyzer with lithium citrate buffers. The method is sensitive to a concentration of 5 nmol, linear in the range of 5-100 nmol, and resolves almost all the bases, nucleosides, nucleotides and their precursors of physiologic importance.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalJournal of Biochemical and Biophysical Methods
Volume1
Issue number4
DOIs
StatePublished - 1979
Externally publishedYes

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Nucleic Acids, Nucleotides, and Nucleosides
Chromatography
Nucleosides
Nucleic Acids
Buffers
Nucleotides
Amino Acids
lithium citrate

Keywords

  • chromatography
  • lithium chloride
  • lithium citrate
  • nucleic acid bases
  • nucleosides
  • nucleotides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precurosors. / Tyagi, Anil K.; Jayaram, Hiremagular N.; Anandaraj, Spurgeon; Taylor, Benjamin; Cooney, David A.

In: Journal of Biochemical and Biophysical Methods, Vol. 1, No. 4, 1979, p. 221-226.

Research output: Contribution to journalArticle

Tyagi, Anil K. ; Jayaram, Hiremagular N. ; Anandaraj, Spurgeon ; Taylor, Benjamin ; Cooney, David A. / Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precurosors. In: Journal of Biochemical and Biophysical Methods. 1979 ; Vol. 1, No. 4. pp. 221-226.
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