Use of G-protein fusions to monitor integral membrane protein-protein interactions in yeast

Kathleen N. Ehrhard, Jörg J. Jacoby, Xin Yuan Fu, Reinhard Jahn, Henrik G. Dohlman

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

The control of protein-protein interactions is a fundamental aspect of cell regulation. Here we describe a new approach to detect the interaction of two proteins in vivo. By this method, one binding partner is an integral membrane protein whereas the other is soluble but fused to a G-protein γ-subunit. If the binding partners interact, G-protein signaling is disrupted. We demonstrate interaction between known binding partners, syntaxin 1 a with neuronal Sec1 (nSec1), and the fibroblast-derived growth factor receptor 3 (FGFR3) with SNT-1. In addition, we describe a genetic screen to identify nSec1 mutants that are expressed normally, but are no longer able to bind to syntaxin 1a. This provides a convenient method to study interactions of integral membrane proteins, a class of molecules that has been difficult to study by existing biochemical or genetic methods.

Original languageEnglish (US)
Pages (from-to)1075-1079
Number of pages5
JournalNature Biotechnology
Volume18
Issue number10 SUPPL.
DOIs
StatePublished - Jan 1 2000

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Ehrhard, K. N., Jacoby, J. J., Fu, X. Y., Jahn, R., & Dohlman, H. G. (2000). Use of G-protein fusions to monitor integral membrane protein-protein interactions in yeast. Nature Biotechnology, 18(10 SUPPL.), 1075-1079. https://doi.org/10.1038/80274