Use of inhibitory (anti-catalytic) antibodies to study extracellular proteolysis

Hanning Birkedal-Hansen, Bente Birkedal-Hansen, L. Windsor, Hong Yea Lin, Robert E. Taylor, William G I Moore

Research output: Contribution to journalArticle

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Abstract

The specific role of proteolytic enzymes in the degradation by live cells of fibrillar model matrices (fibrin, collagen) was studied using monoclonal and polyclonal inhibitory (anti-catalytic) antibodies. Dissolution of fibrin by plasminogen-supplemented human HT-1080 cells was blocked by (1) omission of plasminogen, (2) inhibitory anti-plasmin antibody, and (3) inhibitory anti-u-PA antibody but not by non-inhibitory control antibodies. Using a similar approach, it was shown that the dissolution of reconstituted type I collagen fibrils by trypsin-supplemented live human skin fibroblasts was blocked by inhibitory antibodies to fibroblast-type procollagenase but not by non-inhibitory control antibodies. These findings permit us to deduce that, at least in culture, the dissolution of fibrin by plasminogen-supplemented HT-1080 cells was mediated by plasminogen-assisted proteolysis which entailed the extracellular conversion of plasminogen to plasmin by cell-derived u-PA, and that the dissolution of collagen fibrils by trypsin-supplemented skin fibroblasts was mediated by a collagenase-dependent pathway.

Original languageEnglish (US)
Pages (from-to)211-224
Number of pages14
JournalImmunological Investigations
Volume18
Issue number1-4
DOIs
StatePublished - 1989
Externally publishedYes

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Catalytic Antibodies
Plasminogen
Proteolysis
Anti-Idiotypic Antibodies
Fibrin
Antibodies
Fibroblasts
Fibrinolysin
Trypsin
Collagen
Skin
Collagenases
Collagen Type I
Peptide Hydrolases

ASJC Scopus subject areas

  • Immunology

Cite this

Birkedal-Hansen, H., Birkedal-Hansen, B., Windsor, L., Lin, H. Y., Taylor, R. E., & Moore, W. G. I. (1989). Use of inhibitory (anti-catalytic) antibodies to study extracellular proteolysis. Immunological Investigations, 18(1-4), 211-224. https://doi.org/10.3109/08820138909112238

Use of inhibitory (anti-catalytic) antibodies to study extracellular proteolysis. / Birkedal-Hansen, Hanning; Birkedal-Hansen, Bente; Windsor, L.; Lin, Hong Yea; Taylor, Robert E.; Moore, William G I.

In: Immunological Investigations, Vol. 18, No. 1-4, 1989, p. 211-224.

Research output: Contribution to journalArticle

Birkedal-Hansen, H, Birkedal-Hansen, B, Windsor, L, Lin, HY, Taylor, RE & Moore, WGI 1989, 'Use of inhibitory (anti-catalytic) antibodies to study extracellular proteolysis', Immunological Investigations, vol. 18, no. 1-4, pp. 211-224. https://doi.org/10.3109/08820138909112238
Birkedal-Hansen, Hanning ; Birkedal-Hansen, Bente ; Windsor, L. ; Lin, Hong Yea ; Taylor, Robert E. ; Moore, William G I. / Use of inhibitory (anti-catalytic) antibodies to study extracellular proteolysis. In: Immunological Investigations. 1989 ; Vol. 18, No. 1-4. pp. 211-224.
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