Use of magnetic stimulation to elicit motor evoked potentials, somatosensory evoked potentials, and H-reflexes in non-sedated rodents

Yi Ping Zhang, Lisa B E Shields, Yongjie Zhang, Jiong Pei, Xiao-Ming Xu, Rachel Hoskins, Jun Cai, Meng Sheng Qiu, David S K Magnuson, Darlene A. Burke, Christopher B. Shields

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Assessment of locomotor function of rodents may be supplemented using electrophysiological tests which monitor the integrity of ascending and descending tracts as well as the focal circuitry of the spinal cord in non-sedated rodents. Magnetically induced SSEPs (M-SSEPs) were elicited in rats by activating the hindpaw using magnetic stimulation (MS). M-SSEP response latencies were slightly longer than those elicited by electrical stimulation. M-SSEPs were eliminated following selective dorsal column lacerations of the spinal cord, indicating that they were transmitted via this tract. Magnetically induced motor evoked potentials (M-MEPs) were elicited in mice following transcranial MS and recorded from the gastrocnemius muscles. M-MEPs performed on myelin deficient mice demonstrated longer onset latencies and smaller amplitudes than in wild-type mice. Magnetically induced H-reflexes (MH-reflexes) which assess local circuitry in the lumbosacral area of the spinal cord were performed in rats. This response disappeared following an L3 contusion spinal cord injury, however, kainic acid (KA) injection at L3, known to selectively destroy interneurons, caused a shorter latency and an increase in the amplitude of the MH-reflex. M-SSEPs and MH-reflexes in rats and M-MEPs in mice compliment locomotor evaluation in assessing the functional integrity of the spinal cord under normal and pathological conditions in the non-sedated animal.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalJournal of Neuroscience Methods
Volume165
Issue number1
DOIs
StatePublished - Sep 15 2007
Externally publishedYes

Fingerprint

H-Reflex
Motor Evoked Potentials
Somatosensory Evoked Potentials
Rodentia
Spinal Cord
Spinal Cord Injuries
Transcranial Magnetic Stimulation
Contusions
Kainic Acid
Interneurons
Myelin Sheath
Electric Stimulation
Reaction Time
Skeletal Muscle
Injections

Keywords

  • Electric stimulation
  • Hoffman reflex
  • Magnetic stimulation
  • Motor evoked potentials
  • Somatosensory evoked potentials

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Use of magnetic stimulation to elicit motor evoked potentials, somatosensory evoked potentials, and H-reflexes in non-sedated rodents. / Zhang, Yi Ping; Shields, Lisa B E; Zhang, Yongjie; Pei, Jiong; Xu, Xiao-Ming; Hoskins, Rachel; Cai, Jun; Qiu, Meng Sheng; Magnuson, David S K; Burke, Darlene A.; Shields, Christopher B.

In: Journal of Neuroscience Methods, Vol. 165, No. 1, 15.09.2007, p. 9-17.

Research output: Contribution to journalArticle

Zhang, YP, Shields, LBE, Zhang, Y, Pei, J, Xu, X-M, Hoskins, R, Cai, J, Qiu, MS, Magnuson, DSK, Burke, DA & Shields, CB 2007, 'Use of magnetic stimulation to elicit motor evoked potentials, somatosensory evoked potentials, and H-reflexes in non-sedated rodents', Journal of Neuroscience Methods, vol. 165, no. 1, pp. 9-17. https://doi.org/10.1016/j.jneumeth.2007.05.021
Zhang, Yi Ping ; Shields, Lisa B E ; Zhang, Yongjie ; Pei, Jiong ; Xu, Xiao-Ming ; Hoskins, Rachel ; Cai, Jun ; Qiu, Meng Sheng ; Magnuson, David S K ; Burke, Darlene A. ; Shields, Christopher B. / Use of magnetic stimulation to elicit motor evoked potentials, somatosensory evoked potentials, and H-reflexes in non-sedated rodents. In: Journal of Neuroscience Methods. 2007 ; Vol. 165, No. 1. pp. 9-17.
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