Use of merocyanine 540 for the isolation of quiescent, primitive human bone marrow hematopoietic progenitor cells

R. E. Pyatt, L. L. Jenski, R. Allen, K. Cornetta, R. Abonour, C. M. Traycoff, E. F. Srour

Research output: Contribution to journalArticle

2 Scopus citations


Merocyanine 540 (MC540) is a membrane probe that inserts preferentially into loosely packed domains in the phospholipid bilayer of intact cells. Previous experiments have demonstrated that MC540 will bind to human bone marrow (BM) hematopoietic progenitor cells (HPC). Fractions of mononuclear BM cells expressing high MC540 fluorescence have been shown to be enriched for myeloid progenitors and cells residing in the S/G2 + M phases of the cell cycle. We rationalized that MC540 uptake could be used to distinguish between quiescent and metabolically active cells and, therefore, to fractionate normal and leukemic BM cells and normal mobilized peripheral blood (MPB) cells into functionally distinct groups of progenitors. BM and MPB cells were separated into fractions ranging in fluorescence from MC540(Bright) to MC540(Dim). Cell cycle analysis of these fractions revealed that the MC540(Dim) fraction of normal and CML BM CD34+ cells constituted the most quiescent fraction, and the MC540(Bright) fractions from these cell types contained the most actively cycling cells. However, no differences in the percentage of cells in G0/G1 were observed between MC540(Bright) and MC540(Dim) fractions of MPB CD34+ cells. To investigate if these cell cycle status differences translated into distinct functional properties, the hematopoietic potential of BM CD34+MC540(Bright) and CD34+MC540(Dim) cell fractions was analyzed in vitro in long-term BM cultures and limiting dilution analysis (LDA) assays. CD34+MC540(Dim) cells produced more total and committed progenitor cells in long-term cultures than did the CD34+MC540(Bright) fraction. The CD34+MC540(Dim) fraction also contained a 2-fold higher number of long-term hematopoietic culture-initiating cells (LTHCIC) than the CD34+MC540(Bright) fraction, as defined by LDA assays. These data demonstrate that MC540 can be a useful probe for the isolation of primitive HPC from some hematopoietic tissues and may assist in monitoring structural changes in the phospholipid bilayer during proliferation and differentiation of HPC.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalJournal of Hematotherapy
Issue number2
StatePublished - 1999

ASJC Scopus subject areas

  • Immunology
  • Hematology

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