Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus

M. R. Tarver, R. E. Shade, R. D. Tarver, Yun Liang, G. Krishnamurthi, B. R. Pittendrigh, L. L. Murdock

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The cowpea bruchid, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchini), is a worldwide pest of stored cowpea grain [Vigna unguiculata (L.) Walp. (Leguminosae)], causing tens of millions of dollars' worth of damage annually. One method of managing this pest involves planting cultivars whose seeds are resistant to bruchids. Despite extensive research, we still do not know the mechanism by which TVu 2027, the original resistant cowpea line, resists C. maculatus. Using micro-CAT (computerized axial tomography) scan imaging, we visualized the feeding patterns of bruchids living and growing within cowpea seeds. We present evidence that an interior zone in TVu 2027 seeds is responsible for the high mortality and developmental delays experienced by avirulent larval bruchids. We observed that both virulent as well as avirulent bruchid strains have different feeding patterns in TVu 2027 seeds compared to susceptible seeds. It appears that the resistance factor is most concentrated in a zone in each cotyledon adjacent to the air space separating the two seed halves.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalEntomologia Experimentalis et Applicata
Volume118
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Callosobruchus maculatus
cowpeas
seed
Bruchidae
seeds
pests
tomography
Vigna unguiculata
Chrysomelidae
Fabaceae
cotyledons
cultivar
image analysis
planting
Coleoptera
mortality
air
damage
cultivars

Keywords

  • Chrysomelidae
  • Coleoptera
  • Computerized axial tomography
  • Cowpea bruchid
  • Host-plant resistance
  • TVu 2027

ASJC Scopus subject areas

  • Insect Science

Cite this

Tarver, M. R., Shade, R. E., Tarver, R. D., Liang, Y., Krishnamurthi, G., Pittendrigh, B. R., & Murdock, L. L. (2006). Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus. Entomologia Experimentalis et Applicata, 118(1), 33-39. https://doi.org/10.1111/j.1570-7458.2006.00360.x

Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus. / Tarver, M. R.; Shade, R. E.; Tarver, R. D.; Liang, Yun; Krishnamurthi, G.; Pittendrigh, B. R.; Murdock, L. L.

In: Entomologia Experimentalis et Applicata, Vol. 118, No. 1, 01.2006, p. 33-39.

Research output: Contribution to journalArticle

Tarver, MR, Shade, RE, Tarver, RD, Liang, Y, Krishnamurthi, G, Pittendrigh, BR & Murdock, LL 2006, 'Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus', Entomologia Experimentalis et Applicata, vol. 118, no. 1, pp. 33-39. https://doi.org/10.1111/j.1570-7458.2006.00360.x
Tarver, M. R. ; Shade, R. E. ; Tarver, R. D. ; Liang, Yun ; Krishnamurthi, G. ; Pittendrigh, B. R. ; Murdock, L. L. / Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus. In: Entomologia Experimentalis et Applicata. 2006 ; Vol. 118, No. 1. pp. 33-39.
@article{b973a97cf51f4438801af296a6be0d3b,
title = "Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus",
abstract = "The cowpea bruchid, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchini), is a worldwide pest of stored cowpea grain [Vigna unguiculata (L.) Walp. (Leguminosae)], causing tens of millions of dollars' worth of damage annually. One method of managing this pest involves planting cultivars whose seeds are resistant to bruchids. Despite extensive research, we still do not know the mechanism by which TVu 2027, the original resistant cowpea line, resists C. maculatus. Using micro-CAT (computerized axial tomography) scan imaging, we visualized the feeding patterns of bruchids living and growing within cowpea seeds. We present evidence that an interior zone in TVu 2027 seeds is responsible for the high mortality and developmental delays experienced by avirulent larval bruchids. We observed that both virulent as well as avirulent bruchid strains have different feeding patterns in TVu 2027 seeds compared to susceptible seeds. It appears that the resistance factor is most concentrated in a zone in each cotyledon adjacent to the air space separating the two seed halves.",
keywords = "Chrysomelidae, Coleoptera, Computerized axial tomography, Cowpea bruchid, Host-plant resistance, TVu 2027",
author = "Tarver, {M. R.} and Shade, {R. E.} and Tarver, {R. D.} and Yun Liang and G. Krishnamurthi and Pittendrigh, {B. R.} and Murdock, {L. L.}",
year = "2006",
month = "1",
doi = "10.1111/j.1570-7458.2006.00360.x",
language = "English",
volume = "118",
pages = "33--39",
journal = "Entomologia Experimentalis et Applicata",
issn = "0013-8703",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Use of micro-CAT scans to understand cowpea seed resistance to Callosobruchus maculatus

AU - Tarver, M. R.

AU - Shade, R. E.

AU - Tarver, R. D.

AU - Liang, Yun

AU - Krishnamurthi, G.

AU - Pittendrigh, B. R.

AU - Murdock, L. L.

PY - 2006/1

Y1 - 2006/1

N2 - The cowpea bruchid, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchini), is a worldwide pest of stored cowpea grain [Vigna unguiculata (L.) Walp. (Leguminosae)], causing tens of millions of dollars' worth of damage annually. One method of managing this pest involves planting cultivars whose seeds are resistant to bruchids. Despite extensive research, we still do not know the mechanism by which TVu 2027, the original resistant cowpea line, resists C. maculatus. Using micro-CAT (computerized axial tomography) scan imaging, we visualized the feeding patterns of bruchids living and growing within cowpea seeds. We present evidence that an interior zone in TVu 2027 seeds is responsible for the high mortality and developmental delays experienced by avirulent larval bruchids. We observed that both virulent as well as avirulent bruchid strains have different feeding patterns in TVu 2027 seeds compared to susceptible seeds. It appears that the resistance factor is most concentrated in a zone in each cotyledon adjacent to the air space separating the two seed halves.

AB - The cowpea bruchid, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchini), is a worldwide pest of stored cowpea grain [Vigna unguiculata (L.) Walp. (Leguminosae)], causing tens of millions of dollars' worth of damage annually. One method of managing this pest involves planting cultivars whose seeds are resistant to bruchids. Despite extensive research, we still do not know the mechanism by which TVu 2027, the original resistant cowpea line, resists C. maculatus. Using micro-CAT (computerized axial tomography) scan imaging, we visualized the feeding patterns of bruchids living and growing within cowpea seeds. We present evidence that an interior zone in TVu 2027 seeds is responsible for the high mortality and developmental delays experienced by avirulent larval bruchids. We observed that both virulent as well as avirulent bruchid strains have different feeding patterns in TVu 2027 seeds compared to susceptible seeds. It appears that the resistance factor is most concentrated in a zone in each cotyledon adjacent to the air space separating the two seed halves.

KW - Chrysomelidae

KW - Coleoptera

KW - Computerized axial tomography

KW - Cowpea bruchid

KW - Host-plant resistance

KW - TVu 2027

UR - http://www.scopus.com/inward/record.url?scp=33645050377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645050377&partnerID=8YFLogxK

U2 - 10.1111/j.1570-7458.2006.00360.x

DO - 10.1111/j.1570-7458.2006.00360.x

M3 - Article

AN - SCOPUS:33645050377

VL - 118

SP - 33

EP - 39

JO - Entomologia Experimentalis et Applicata

JF - Entomologia Experimentalis et Applicata

SN - 0013-8703

IS - 1

ER -