Use of the pH-sensitive dye BCECF to study pH regulation in cultured human kidney proximal tubule cells

J. A. Bergman, J. A. McAteer, Andrew Evan, M. Soleimani

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This protocol describes the use of the pH-sensitive, intracellularly trapped dye 2′,7′-bis (2-carboxyethyl), 5 (and -6) carboxyfluorescein (BCECF), to characterize the pH regulating mechanisms in cultured human kidney proximal tubule cells. This is a reliable method for intracellular pH measurements and is applicable to single cells, cell suspensions, and confluent cultures.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalJournal of Tissue Culture Methods
Volume13
Issue number3
DOIs
StatePublished - 1991

Fingerprint

Proximal Kidney Tubule
Suspensions
Coloring Agents
Dyes
2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein
2-(2-(2-chloro-3-(2-(3,3-dimethyl-5-sulfo-1-(4-sulfo-butyl)-3H-indol-2-yl)-vinyl)-cyclohex-2-enylidene)-ethylidene)-3,3-dimethyl-1-(4-sulfo-butyl)-2,3-dihydro-1H-indole-5-carboxylic acid
6-carboxyfluorescein

Keywords

  • BCECF
  • cell culture
  • human kidney
  • NH(Formula presented.)
  • pH
  • proximal tubule

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Use of the pH-sensitive dye BCECF to study pH regulation in cultured human kidney proximal tubule cells. / Bergman, J. A.; McAteer, J. A.; Evan, Andrew; Soleimani, M.

In: Journal of Tissue Culture Methods, Vol. 13, No. 3, 1991, p. 205-209.

Research output: Contribution to journalArticle

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