Aplicación del método de la reacción en cadena de la polimerasa al análisis genético de la fibrosis quística.

Translated title of the contribution: Use of the polymerase chain reaction technic in the genetic analysis of cystic fibrosis

M. Chillón, T. Casals, F. J. Giménez, H. Badía, N. Morral, A. Bosch, V. Nunes, X. Estivill

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The development of polymerase chain reaction (PCR) which allows the specific amplification of DNA sequences has improved considerably the genetic analysis of hereditary diseases. We present here the application of this new technique to the genetic analysis of cystic fibrosis (CF), the most frequent severe genetic disease in caucasians. We have amplified four sequences containing polymorphisms linked to the CF gene (CS.7, KM.19, MP6d-9 and J3.11), and analysed the amplified products with restriction enzymes. Complete concordance was found with classical Southern methods, allowing the application of PCR to routine CF family studies.

Translated title of the contributionUse of the polymerase chain reaction technic in the genetic analysis of cystic fibrosis
Original languageSpanish
Pages (from-to)444-447
Number of pages4
JournalMedicina clinica
Volume94
Issue number12
StatePublished - Mar 31 1990
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Use of the polymerase chain reaction technic in the genetic analysis of cystic fibrosis'. Together they form a unique fingerprint.

Cite this