Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland

F. P. O'Malley, David Grignon, D. T. Shum

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

There are a number of benign, small-acinar lesions in the prostate gland that may be difficult to differentiate from small-acinar adenocarcinoma. An important diagnostic criterion in this differentiation is the loss of the basal layer in small-acinar adenocarcinoma and its preservation in benign conditions. A monoclonal antibody to high-molecular-weight cytokeratins (34 βE12) has been shown to stain these basal cells preferentially. To assess the usefulness of this antibody in distinguishing benign from malignant small-acinar lesions, we examined 21 cases of small-acinar adenocarcinoma and 47 examples of benign lesions, which included atypical adenomatous hyperplasia, atrophy, post-sclerotic hyperplasia, basal cell hyperplasia, and fibroepithelial nodule. Positive staining with 34 βEl2 was seen in 13/13 cases of atypical adenomatous hyperplasia, although in some cases the staining was weak and focal. Positivity with 34 βE12 was also demonstrated in all other benign lesions studied. All 21 cases of small-acinar adenocarcinoma showed no reactivity with 34 βEl2. The results suggest that 34 βE12 is of value in distinguishing between well-differentiated, small-acinar prostatic adenocarcinoma and its mimics. However, care is needed in interpretation of staining in formalin-fixed material due to the variable reactivity, particularly in cases of atypical adenomatous hyperplasia.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Volume417
Issue number3
DOIs
StatePublished - May 1990
Externally publishedYes

Fingerprint

Keratins
Hyperplasia
Prostate
Adenocarcinoma
Differential Diagnosis
Molecular Weight
Staining and Labeling
Formaldehyde
Atrophy
Coloring Agents
Monoclonal Antibodies
Antibodies

Keywords

  • High-molecular-weight cytokeratin
  • Prostatic adenocarcinoma
  • Prostatic basal cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

@article{ecc368f530c84586922d4e9e7bb1cde6,
title = "Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland",
abstract = "There are a number of benign, small-acinar lesions in the prostate gland that may be difficult to differentiate from small-acinar adenocarcinoma. An important diagnostic criterion in this differentiation is the loss of the basal layer in small-acinar adenocarcinoma and its preservation in benign conditions. A monoclonal antibody to high-molecular-weight cytokeratins (34 βE12) has been shown to stain these basal cells preferentially. To assess the usefulness of this antibody in distinguishing benign from malignant small-acinar lesions, we examined 21 cases of small-acinar adenocarcinoma and 47 examples of benign lesions, which included atypical adenomatous hyperplasia, atrophy, post-sclerotic hyperplasia, basal cell hyperplasia, and fibroepithelial nodule. Positive staining with 34 βEl2 was seen in 13/13 cases of atypical adenomatous hyperplasia, although in some cases the staining was weak and focal. Positivity with 34 βE12 was also demonstrated in all other benign lesions studied. All 21 cases of small-acinar adenocarcinoma showed no reactivity with 34 βEl2. The results suggest that 34 βE12 is of value in distinguishing between well-differentiated, small-acinar prostatic adenocarcinoma and its mimics. However, care is needed in interpretation of staining in formalin-fixed material due to the variable reactivity, particularly in cases of atypical adenomatous hyperplasia.",
keywords = "High-molecular-weight cytokeratin, Prostatic adenocarcinoma, Prostatic basal cells",
author = "O'Malley, {F. P.} and David Grignon and Shum, {D. T.}",
year = "1990",
month = "5",
doi = "10.1007/BF01600133",
language = "English (US)",
volume = "417",
pages = "191--196",
journal = "Virchows Archiv",
issn = "0945-6317",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland

AU - O'Malley, F. P.

AU - Grignon, David

AU - Shum, D. T.

PY - 1990/5

Y1 - 1990/5

N2 - There are a number of benign, small-acinar lesions in the prostate gland that may be difficult to differentiate from small-acinar adenocarcinoma. An important diagnostic criterion in this differentiation is the loss of the basal layer in small-acinar adenocarcinoma and its preservation in benign conditions. A monoclonal antibody to high-molecular-weight cytokeratins (34 βE12) has been shown to stain these basal cells preferentially. To assess the usefulness of this antibody in distinguishing benign from malignant small-acinar lesions, we examined 21 cases of small-acinar adenocarcinoma and 47 examples of benign lesions, which included atypical adenomatous hyperplasia, atrophy, post-sclerotic hyperplasia, basal cell hyperplasia, and fibroepithelial nodule. Positive staining with 34 βEl2 was seen in 13/13 cases of atypical adenomatous hyperplasia, although in some cases the staining was weak and focal. Positivity with 34 βE12 was also demonstrated in all other benign lesions studied. All 21 cases of small-acinar adenocarcinoma showed no reactivity with 34 βEl2. The results suggest that 34 βE12 is of value in distinguishing between well-differentiated, small-acinar prostatic adenocarcinoma and its mimics. However, care is needed in interpretation of staining in formalin-fixed material due to the variable reactivity, particularly in cases of atypical adenomatous hyperplasia.

AB - There are a number of benign, small-acinar lesions in the prostate gland that may be difficult to differentiate from small-acinar adenocarcinoma. An important diagnostic criterion in this differentiation is the loss of the basal layer in small-acinar adenocarcinoma and its preservation in benign conditions. A monoclonal antibody to high-molecular-weight cytokeratins (34 βE12) has been shown to stain these basal cells preferentially. To assess the usefulness of this antibody in distinguishing benign from malignant small-acinar lesions, we examined 21 cases of small-acinar adenocarcinoma and 47 examples of benign lesions, which included atypical adenomatous hyperplasia, atrophy, post-sclerotic hyperplasia, basal cell hyperplasia, and fibroepithelial nodule. Positive staining with 34 βEl2 was seen in 13/13 cases of atypical adenomatous hyperplasia, although in some cases the staining was weak and focal. Positivity with 34 βE12 was also demonstrated in all other benign lesions studied. All 21 cases of small-acinar adenocarcinoma showed no reactivity with 34 βEl2. The results suggest that 34 βE12 is of value in distinguishing between well-differentiated, small-acinar prostatic adenocarcinoma and its mimics. However, care is needed in interpretation of staining in formalin-fixed material due to the variable reactivity, particularly in cases of atypical adenomatous hyperplasia.

KW - High-molecular-weight cytokeratin

KW - Prostatic adenocarcinoma

KW - Prostatic basal cells

UR - http://www.scopus.com/inward/record.url?scp=0025290840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025290840&partnerID=8YFLogxK

U2 - 10.1007/BF01600133

DO - 10.1007/BF01600133

M3 - Article

C2 - 1696762

AN - SCOPUS:0025290840

VL - 417

SP - 191

EP - 196

JO - Virchows Archiv

JF - Virchows Archiv

SN - 0945-6317

IS - 3

ER -