Using LC3 to monitor autophagy flux in the retinal pigment epithelium

Ai Ling Wang, Michael E. Boulton, William A. Dunn, Harripriya Vittal Rao, Jun Cai, Thomas J. Lukas, Arthur H. Neufeld

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Autophagy is a highly conserved housekeeping pathway that plays a critical role in the removal of aged or damaged intracellular organelles and their delivery to lysosomes for degradation. 1,2 Autophagy begins with the formation of membranes, arising in part from the endoplasmic reticulum, that elongate and fuse engulfing cytoplasmic constituents into a classic double-membrane bound nascent autophagosome. These early autophagosomes undergo a stepwise maturation process to form the late autophagosome or amphisome that ultimately fuses with a lysosome. Efficient autophagy is dependent on an equilibrium between the formation and elimination of autophagosomes; thus, a deficit in any part of this pathway will cause autophagic dysfunction. Autophagy plays a role in aging and agerelated diseases.1,2,7 However, few studies of autophagy in retinal disease have been reported.

Original languageEnglish (US)
Pages (from-to)1190-1193
Number of pages4
JournalAutophagy
Volume5
Issue number8
DOIs
StatePublished - Nov 16 2009

    Fingerprint

Keywords

  • Age-related macular degeneration
  • Aging
  • Autophagy flux
  • LC3
  • Lysosomes
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Wang, A. L., Boulton, M. E., Dunn, W. A., Rao, H. V., Cai, J., Lukas, T. J., & Neufeld, A. H. (2009). Using LC3 to monitor autophagy flux in the retinal pigment epithelium. Autophagy, 5(8), 1190-1193. https://doi.org/10.4161/auto.5.8.10087