Using quantitative intravital multiphoton microscopy to dissect hepatic transport in rats

Kenneth Dunn, Jennifer C. Ryan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hepatic solute transport is a complex process whose disruption is associated with liver disease and drug-induced liver injury. Intravital multiphoton fluorescence excitation microscopy provides the spatial and temporal resolution necessary to characterize hepatic transport at the level of individual hepatocytes in vivo and thus to identify the mechanisms and cellular consequences of cholestasis. Here we present an overview of the use of fluorescence microscopy for studies of hepatic transport in living animals, and describe how we have combined methods of intravital microscopy and digital image analysis to dissect the effects of drugs and pathological conditions on the function of hepatic transporters in vivo.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - Feb 1 2017

Fingerprint

Liver
Rats
Microscopic examination
Solute transport
Fluorescence microscopy
Pharmaceutical Preparations
Image analysis
Multiphoton Fluorescence Microscopy
Animals
Fluorescence
Chemical and Drug Induced Liver Injury
Cholestasis
Fluorescence Microscopy
Liver Diseases
Hepatocytes
Intravital Microscopy

Keywords

  • Cholestasis
  • Digital image analysis
  • Fluorescence microscopy
  • In vivo imaging
  • Intravital microscopy
  • Liver injury

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Using quantitative intravital multiphoton microscopy to dissect hepatic transport in rats. / Dunn, Kenneth; Ryan, Jennifer C.

In: Methods, 01.02.2017.

Research output: Contribution to journalArticle

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