Using quantitative intravital multiphoton microscopy to dissect hepatic transport in rats

Kenneth W. Dunn, Jennifer C. Ryan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Hepatic solute transport is a complex process whose disruption is associated with liver disease and drug-induced liver injury. Intravital multiphoton fluorescence excitation microscopy provides the spatial and temporal resolution necessary to characterize hepatic transport at the level of individual hepatocytes in vivo and thus to identify the mechanisms and cellular consequences of cholestasis. Here we present an overview of the use of fluorescence microscopy for studies of hepatic transport in living animals, and describe how we have combined methods of intravital microscopy and digital image analysis to dissect the effects of drugs and pathological conditions on the function of hepatic transporters in vivo.

Original languageEnglish (US)
Pages (from-to)40-51
Number of pages12
JournalMethods
Volume128
DOIs
StatePublished - Sep 1 2017

Keywords

  • Cholestasis
  • Digital image analysis
  • Fluorescence microscopy
  • In vivo imaging
  • Intravital microscopy
  • Liver injury

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Using quantitative intravital multiphoton microscopy to dissect hepatic transport in rats'. Together they form a unique fingerprint.

Cite this