Posttranslational modifi cations have long been known to play an essential role in the regulation of NF-κB activity. In the past few years, in addition to more traditional modifi cations such as phosphorylation, the p65 subunit of NF-κB has been found to be methylated at multiple sites. Here, we describe procedures for using immunoprecipitation and mass spectrometry to identify the methylation sites of p65.
- Mass spectrometry
- Posttranslational modifi cation
ASJC Scopus subject areas
- Molecular Biology