Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-κB

Tao Lu, George R. Stark

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Posttranslational modifi cations have long been known to play an essential role in the regulation of NF-κB activity. In the past few years, in addition to more traditional modifi cations such as phosphorylation, the p65 subunit of NF-κB has been found to be methylated at multiple sites. Here, we describe procedures for using immunoprecipitation and mass spectrometry to identify the methylation sites of p65.

Original languageEnglish (US)
Pages (from-to)383-393
Number of pages11
JournalMethods in Molecular Biology
Volume1280
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Immunoprecipitation
  • Mass spectrometry
  • Methylation
  • NF-κB
  • P65
  • Posttranslational modifi cation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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