Using viral species specificity to define a critical protein/RNA interaction surface

Glen A. Coburn, Heather L. Wiegand, Yibin Kang, Dona N. Ho, Millie Georgiadis, Bryan R. Cullen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Tap protein mediates the sequence-specific nuclear export of mRNAs bearing the retroviral constitutive transport element (CTE) and also plays a critical role in the sequence nonspecific export of cellular mRNAs. Previously, we have demonstrated that CTE function displays species specificity, that is, the CTE functions in human but not quail cells. Here, we demonstrate that quail Tap fails to support CTE function because it cannot bind the CTE. However, changing a single residue in quail Tap, glutamine 246, to arginine, the residue found in human Tap, rescues both CTE function and CTE binding. This residue, which is located on the exterior of a recently reported molecular structure of Tap, defines a surface on Tap that is critical for CTE binding. These data emphasize the potential importance of cross-species genetic complementation in the identification and characterization of cellular factors that are critical for different aspects of viral replication.

Original languageEnglish (US)
Pages (from-to)1194-1205
Number of pages12
JournalGenes and Development
Volume15
Issue number10
DOIs
StatePublished - May 15 2001
Externally publishedYes

Fingerprint

Species Specificity
Quail
RNA
Genetic Crosses
Messenger RNA
Proteins
Cell Nucleus Active Transport
Molecular Structure
Glutamine
Arginine

Keywords

  • Gene regulation
  • Nuclear export
  • Retrovirus
  • RNA binding

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Using viral species specificity to define a critical protein/RNA interaction surface. / Coburn, Glen A.; Wiegand, Heather L.; Kang, Yibin; Ho, Dona N.; Georgiadis, Millie; Cullen, Bryan R.

In: Genes and Development, Vol. 15, No. 10, 15.05.2001, p. 1194-1205.

Research output: Contribution to journalArticle

Coburn, Glen A. ; Wiegand, Heather L. ; Kang, Yibin ; Ho, Dona N. ; Georgiadis, Millie ; Cullen, Bryan R. / Using viral species specificity to define a critical protein/RNA interaction surface. In: Genes and Development. 2001 ; Vol. 15, No. 10. pp. 1194-1205.
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