UVB Generates Microvesicle Particle Release in Part Due to Platelet-activating Factor Signaling

Ji C. Bihl, Christine M. Rapp, Yanfang Chen, Jeffrey Travers

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The lipid mediator platelet-activating factor (PAF) and oxidized glycerophosphocholine PAF agonists produced by ultraviolet B (UVB) have been demonstrated to play a pivotal role in UVB-mediated processes, from acute inflammation to delayed systemic immunosuppression. Recent studies have provided evidence that microvesicle particles (MVPs) are released from cells in response to various signals including stressors. Importantly, these small membrane fragments can interact with various cell types by delivering bioactive molecules. The present studies were designed to test if UVB radiation can generate MVP release from epithelial cells, and the potential role of PAF receptor (PAF-R) signaling in this process. We demonstrate that UVB irradiation of the human keratinocyte-derived cell line HaCaT resulted in the release of MVPs. Similarly, treatment of HaCaT cells with the PAF-R agonist carbamoyl PAF also generated equivalent amounts of MVP release. Of note, pretreatment of HaCaT cells with antioxidants blocked MVP release from UVB but not PAF-R agonist N-methyl carbamyl PAF (CPAF). Importantly, UVB irradiation of the PAF-R-negative human epithelial cell line KB and KB transduced with functional PAF-Rs resulted in MVP release only in PAF-R-positive cells. These studies demonstrate that UVB can generate MVPs in vitro and that PAF-R signaling appears important in this process.

Original languageEnglish (US)
JournalPhotochemistry and Photobiology
DOIs
StateAccepted/In press - 2016
Externally publishedYes

Fingerprint

Platelet Activating Factor
platelets
Cells
cells
cultured cells
Epithelial Cells
Irradiation
Cell Line
immunosuppression
Keratinocytes
irradiation
Immunosuppression
antioxidants
activity (biology)
pretreatment
Antioxidants
lipids
Radiation
Inflammation
Membranes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

UVB Generates Microvesicle Particle Release in Part Due to Platelet-activating Factor Signaling. / Bihl, Ji C.; Rapp, Christine M.; Chen, Yanfang; Travers, Jeffrey.

In: Photochemistry and Photobiology, 2016.

Research output: Contribution to journalArticle

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