UVB-induced activation of NF-κB is regulated by the IGF-1R and dependent on p38 MAPK

Davina A. Lewis, Dan Spandau

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To manage the frequent exposure to carcinogenic UVB wavelengths found in sunlight, keratinocytes have extensive protective measures to handle UVB-induced DNA damage. Recent in vitro evidence and epidemiological data suggest that one possible protective mechanism is dependent on the functional status of the IGF-1R signaling network. A second important signaling pathway regulating the response of keratinocytes to UVB involves the activation of the NF-κB transcription factor. Although it is clear that proper functioning of both the IGF-1R and NF-κB signaling networks are critical for the appropriate response of keratinocytes to UVB irradiation, it is currently uncertain if these two pathways interact. We now demonstrate that the activation of the NF-κB transcription factor by UVB is altered by the functional status of the IGF-1R. In the absence of ligand-activated IGF-1R, UVB-induced NF-κB consisted primarily of p50:p50 homodimers. Furthermore, the p38 kinase MAPK directs the subunit composition of NF-κB following UVB irradiation, most likely in an IGF-1R-dependent manner. We hypothesize that UVB irradiation leads to an activated p38 MAPK that is regulated in an IGF-1R-dependent manner, leading to NF-κB p50:RelA/p65 activation and a survival phenotype. In the absence of ligand-activated IGF-1R, UVB irradiation leads to the induction of NF-κB p50:p50 homodimers and a p38-dependent increased susceptibility to apoptosis.

Original languageEnglish
Pages (from-to)1022-1029
Number of pages8
JournalJournal of Investigative Dermatology
Volume128
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

p38 Mitogen-Activated Protein Kinases
Keratinocytes
Chemical activation
Irradiation
Transcription Factors
Ligands
Sunlight
DNA Damage
Mitogen-Activated Protein Kinase Kinases
Phosphotransferases
Apoptosis
Phenotype
Wavelength
DNA
Chemical analysis
3'-(1-butylphosphoryl)adenosine

ASJC Scopus subject areas

  • Dermatology

Cite this

UVB-induced activation of NF-κB is regulated by the IGF-1R and dependent on p38 MAPK. / Lewis, Davina A.; Spandau, Dan.

In: Journal of Investigative Dermatology, Vol. 128, No. 4, 04.2008, p. 1022-1029.

Research output: Contribution to journalArticle

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