UVB-induced senescence in human keratinocytes requires a functional insulin-like growth factor-1 receptor and p53

Davina A. Lewis, Qiaofang Yi, Jeffrey Travers, Dan Spandau

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

To cope with the frequent exposure to carcinogenic UV B (UVB) wavelengths found in sunlight, keratinocytes have acquired extensive protective measures to handle UVB-induced DNA damage. Recent in vitro and epidemiological data suggest one these protective mechanisms is dependent on the functional status of the insulin-like growth factor-1 receptor (IGF-1R) signaling network in keratinocytes. During the normal UVB response, ligand-activated IGF-1Rs protect keratinocytes from UVB-induced apoptosis; however, as a consequence, these keratinocytes fail to proliferate. This adaptive response of keratinocytes to UVB exposure maintains the protective barrier function of the epidermis while ensuring that UVB-damaged keratinocytes do not replicate DNA mutations. In contrast, when keratinocytes are exposed to UVB in the absence of IGF-1R activation, the keratinocytes are more sensitive to UVB-induced apoptosis, but the surviving keratinocytes retain the capacity to proliferate. This aberrant UVB response represents flawed protection from UVB damage potentially resulting in the malignant transformation of keratinocytes. Using normal human keratinocytes grown in vitro, we have demonstrated that activation of the IGF-1R promotes the premature senescence of UVB-irradiated keratinocytes through increased generation of reactive oxygen species (ROS) and by maintaining the expression of the cyclin-dependent kinase inhibitor p21CDKN1A. Furthermore, IGF-1R-dependent UVB-induced premature senescence required the phosphorylation of p53 serine 46. These data suggest one mechanism of keratinocyte resistance to UVB-induced carcinogenesis involves the induction of IGF-1R-dependent premature senescence.

Original languageEnglish
Pages (from-to)1346-1353
Number of pages8
JournalMolecular Biology of the Cell
Volume19
Issue number4
DOIs
StatePublished - Apr 2008

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Somatomedin Receptors
Keratinocytes
Apoptosis
Cyclin-Dependent Kinases
Sunlight
Epidermis
Serine
DNA Damage

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

UVB-induced senescence in human keratinocytes requires a functional insulin-like growth factor-1 receptor and p53. / Lewis, Davina A.; Yi, Qiaofang; Travers, Jeffrey; Spandau, Dan.

In: Molecular Biology of the Cell, Vol. 19, No. 4, 04.2008, p. 1346-1353.

Research output: Contribution to journalArticle

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