UVB radiation-mediated inhibition of contact hypersensitivity reactions is dependent on the platelet-activating factor system

Qiwei Zhang, Yongxue Yao, Raymond Konger, Anthony L. Sinn, Shanbao Cai, Karen Pollok, Jeffrey Travers

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Through its ability to both induce immunosuppression and act as a carcinogen, UVB radiation plays a major role in cutaneous malignancies. Recent studies have indicated that UVB-mediated inhibition of delayed-type hypersensitivity reactions is mediated, in part, by the lipid mediator platelet-activating factor (PAF). The objective of this study was to further define the mechanism by which UVB inhibits contact hypersensitivity (CHS) reactions. UVB irradiation resulted in an inhibition of subsequent CHS to the chemical DNFB in wild-type, but not in PAF-R-deficient mice. UVB-mediated inhibition of CHS was also blocked by a cyclooxygenase-2 (COX-2) inhibitor or a neutralizing antibody directed against IL-10. UVB irradiation upregulated IL-10 mRNA levels in lymph nodes and spleen only to significant levels in PAF-R-expressing mice. Bone marrow transplantation studies demonstrated that UVB-mediated immunomodulatory effects were dependent on PAF-R-positive bone marrow. These studies suggest that UVB irradiation results in epidermal production of PAF agonists, which then act on PAF-R-positive bone marrow-derived cells to upregulate IL-10 through COX-2-generated prostaglandins.

Original languageEnglish
Pages (from-to)1780-1787
Number of pages8
JournalJournal of Investigative Dermatology
Volume128
Issue number7
DOIs
StatePublished - Jul 2008

Fingerprint

Platelet Activating Factor
Contact Dermatitis
Radiation
Interleukin-10
Bone
Irradiation
Dinitrofluorobenzene
Cyclooxygenase 2 Inhibitors
Delayed Hypersensitivity
Cyclooxygenase 2
Neutralizing Antibodies
Bone Marrow Transplantation
Bone Marrow Cells
Carcinogens
Immunosuppression
Prostaglandins
Up-Regulation
Spleen
Lymph Nodes
Bone Marrow

ASJC Scopus subject areas

  • Dermatology

Cite this

UVB radiation-mediated inhibition of contact hypersensitivity reactions is dependent on the platelet-activating factor system. / Zhang, Qiwei; Yao, Yongxue; Konger, Raymond; Sinn, Anthony L.; Cai, Shanbao; Pollok, Karen; Travers, Jeffrey.

In: Journal of Investigative Dermatology, Vol. 128, No. 7, 07.2008, p. 1780-1787.

Research output: Contribution to journalArticle

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