Vaccination against prostate cancer using a live tissue factor deficient cell line in Lobund-Wistar rats

Julie E. Heinrich, Morris Pollard, William A. Wolter, Zhong Liang, Hui Song, Elliot D. Rosen, Mark A. Suckow

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Reducing expression of the tissue factor gene in prostate adenocarcinoma cells (PAIII) results in a cell line that, in vivo, mimics the growth of wildtype (wt) PAIII. However, instead of continuing to grow and metastasize as wt PAIII tumors do, tissue factor deficient PAIII (TFD PAIII) masses spontaneously regress after several weeks. Although whole cell vaccines are typically inactivated prior to administration to prevent proliferation within the host, numerous studies have suggested that exposure to live, attenuated, whole tumor cells, and the extracellular microenvironment they recruit, increases immunotherapeutic potential. Here, we provide support for this notion, and a strategy through which to implement it, by demonstrating that subcutaneous vaccinations with the TFD PAIII protect the Lobund-Wistar rat against subsequent wt PAIII cell challenge. TFD PAIII immunized rats suffered significantly less metastasis of wt PAIII challenge tumors compared to unvaccinated naïve controls rats. These results offer the intriguing possibility that the TFD PAIII vaccine is an effective system for the prevention and, possibly, the treatment of prostate cancer.

Original languageEnglish (US)
Pages (from-to)725-730
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume56
Issue number5
DOIs
StatePublished - May 1 2007

Keywords

  • Metastasis
  • Prostate cancer
  • Stroma
  • Tissue factor
  • Vaccine
  • Whole-tumor cell

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Oncology

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