Vaccinia virus infection induces dendritic cell maturation but inhibits antigen presentation by MHC class II

Yongxue Yao, Ping Li, Pratibha Singh, Allison T. Thiele, David S. Wilkes, Gourapura J. Renukaradhya, Randy R. Brutkiewicz, Jeffrey B. Travers, Gary D. Luker, Soon Cheol Hong, Janice S. Blum, Cheong Hee Chang

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32 Scopus citations

Abstract

Vaccinia virus (VV) infection is known to inhibit dendritic cells (DC) functions in vitro. Paradoxically, VV is also highly immunogenic and thus has been used as a vaccine. In the present study, we investigated the effects of an in vivo VV infection on DC function by focusing on early innate immunity. Our data indicated that DC are activated upon in vivo VV infection of mice. Splenic DC from VV-infected mice expressed elevated levels of MHC class I and co-stimulatory molecules on their cell surface and exhibited the enhanced potential to produce cytokines upon LPS stimulation. DC from VV-infected mice also expressed a high level of interferon-β. However, a VV infection resulted in the down-regulation of MHC class II expression and the impairment of antigen presentation to CD4 T cells by DC. Thus, during the early stage of a VV infection, although DC are impaired in some of the critical antigen presentation functions, they can promote innate immune defenses against viral infection.

Original languageEnglish (US)
Pages (from-to)92-102
Number of pages11
JournalCellular Immunology
Volume246
Issue number2
DOIs
StatePublished - Apr 1 2007

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Keywords

  • Cytokines
  • Dendritic cell
  • Gene regulation
  • Innate immunity
  • MHC class II
  • Type I interferon
  • Vaccinia virus

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Yao, Y., Li, P., Singh, P., Thiele, A. T., Wilkes, D. S., Renukaradhya, G. J., Brutkiewicz, R. R., Travers, J. B., Luker, G. D., Hong, S. C., Blum, J. S., & Chang, C. H. (2007). Vaccinia virus infection induces dendritic cell maturation but inhibits antigen presentation by MHC class II. Cellular Immunology, 246(2), 92-102. https://doi.org/10.1016/j.cellimm.2007.06.005