Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow

Pratibha Singh, Yongxue Yao, Abigail Weliver, Hal Broxmeyer, Soon Cheol Hong, Cheong Hee Chang

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Successful proliferation and differentiation of hematopoietic progenitor cells in bone marrow (BM) is essential to generate all mature blood cell types, including those involved in the immune response. Although vaccinia virus (VV) is known to induce a strong immune response, the effect of VV infection on hematopoiesis remains largely unknown. Here, we showed that in vivo VV infection results in the expansion of c-KithiSca-1+Lin- (KSL) hematopoietic stem cells. The in vivo expansion of the KSL population requires MyD88 that is a critical adaptor for Toll-like receptor-mediated signaling. Moreover, in BM of VV-infected mice, common myeloid progenitors (CMP) was decreased because of the rapid differentiation of CMP to more mature cells. However, the CMP compartment was not affected by VV infection in the absence of MyD88. The common lymphoid progenitor (CLP) cell population was increased regardless of MyD88 status, suggesting the independent regulation of CMP and CLP compartments by VV infection. VV infection also enhanced the potential of progenitors that preferentially induce the programming of dendritic cell (DC) development toward plasmacytoid DC. Therefore, the host immune response is gearing toward antiviral responses as early as at the precursor level upon VV infection.

Original languageEnglish
Pages (from-to)1009-1016
Number of pages8
JournalStem Cells
Volume26
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Vaccinia virus
Virus Diseases
Bone Marrow
Myeloid Progenitor Cells
Lymphoid Progenitor Cells
Hematopoietic Stem Cells
Dendritic Cells
Toll-Like Receptors
Hematopoiesis
Population
Antiviral Agents
Blood Cells

Keywords

  • Bone marrow
  • Committed progenitors
  • Dendritic cells
  • Differentiation

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine
  • Medicine(all)

Cite this

Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow. / Singh, Pratibha; Yao, Yongxue; Weliver, Abigail; Broxmeyer, Hal; Hong, Soon Cheol; Chang, Cheong Hee.

In: Stem Cells, Vol. 26, No. 4, 04.2008, p. 1009-1016.

Research output: Contribution to journalArticle

Singh, Pratibha ; Yao, Yongxue ; Weliver, Abigail ; Broxmeyer, Hal ; Hong, Soon Cheol ; Chang, Cheong Hee. / Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow. In: Stem Cells. 2008 ; Vol. 26, No. 4. pp. 1009-1016.
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