Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse

Kathryn M. Ziegler, Terence E. Wade, Sue Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas Zyromski

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Many experimental models of acute pancreatitis suffer from lack of clinical relevance. We sought to validate a recently reported murine model of acute pancreatitis that more closely represents the physiology of human biliary pancreatitis. Methods: Mice (C57BL/6J n=6 and CF-1 n=8) underwent infusion of 50μl of 5% sodium taurocholate (NaT) or 50μl of normal saline (NaCl) directly into the pancreatic duct. Twenty-four hours later, pancreatitis severity was graded histologically by three independent observers, and pancreatic tissue concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined by ELISA. Results: Twenty four hours after retrograde injection, the total pancreatitis score was significantly greater in mice infused with NaT than in those infused with NaCl (6.3 ± 1.2 vs. 1.2 ± 0.4, p<0.05). In addition, the inflammatory mediators IL-6 and MCP-1 were increased in the NaT group relative to the NaCl group. Discussion: Retrograde pancreatic duct infusion of sodium taurocholate induces acute pancreatitis in the mouse. This model is likely representative of human biliary pancreatitis pathophysiology, and therefore provides a powerful tool with which to elucidate basic mechanisms underlying the pathogenesis of acute pancreatitis.

Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalAmerican Journal of Translational Research
Volume3
Issue number2
StatePublished - 2011

Fingerprint

Pancreatitis
Taurocholic Acid
Theoretical Models
Chemokine CCL2
Ducts
Interleukin-6
Physiology
Pancreatic Ducts
Tissue
Inbred C57BL Mouse
Enzyme-Linked Immunosorbent Assay
Injections

Keywords

  • Acute pancreatitis
  • Animal model
  • Murine
  • Retrograde injection
  • Sodium taurocholate

ASJC Scopus subject areas

  • Medicine(all)
  • Cancer Research
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Ziegler, K. M., Wade, T. E., Wang, S., Swartz-Basile, D. A., Pitt, H. A., & Zyromski, N. (2011). Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse. American Journal of Translational Research, 3(2), 159-165.

Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse. / Ziegler, Kathryn M.; Wade, Terence E.; Wang, Sue; Swartz-Basile, Deborah A.; Pitt, Henry A.; Zyromski, Nicholas.

In: American Journal of Translational Research, Vol. 3, No. 2, 2011, p. 159-165.

Research output: Contribution to journalArticle

Ziegler, KM, Wade, TE, Wang, S, Swartz-Basile, DA, Pitt, HA & Zyromski, N 2011, 'Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse', American Journal of Translational Research, vol. 3, no. 2, pp. 159-165.
Ziegler, Kathryn M. ; Wade, Terence E. ; Wang, Sue ; Swartz-Basile, Deborah A. ; Pitt, Henry A. ; Zyromski, Nicholas. / Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse. In: American Journal of Translational Research. 2011 ; Vol. 3, No. 2. pp. 159-165.
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