Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse

Kathryn M. Ziegler, Terence E. Wade, Sue Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas J. Zyromski

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: Many experimental models of acute pancreatitis suffer from lack of clinical relevance. We sought to validate a recently reported murine model of acute pancreatitis that more closely represents the physiology of human biliary pancreatitis. Methods: Mice (C57BL/6J n=6 and CF-1 n=8) underwent infusion of 50μl of 5% sodium taurocholate (NaT) or 50μl of normal saline (NaCl) directly into the pancreatic duct. Twenty-four hours later, pancreatitis severity was graded histologically by three independent observers, and pancreatic tissue concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined by ELISA. Results: Twenty four hours after retrograde injection, the total pancreatitis score was significantly greater in mice infused with NaT than in those infused with NaCl (6.3 ± 1.2 vs. 1.2 ± 0.4, p<0.05). In addition, the inflammatory mediators IL-6 and MCP-1 were increased in the NaT group relative to the NaCl group. Discussion: Retrograde pancreatic duct infusion of sodium taurocholate induces acute pancreatitis in the mouse. This model is likely representative of human biliary pancreatitis pathophysiology, and therefore provides a powerful tool with which to elucidate basic mechanisms underlying the pathogenesis of acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalAmerican Journal of Translational Research
Volume3
Issue number2
StatePublished - Mar 28 2011

Keywords

  • Acute pancreatitis
  • Animal model
  • Murine
  • Retrograde injection
  • Sodium taurocholate

ASJC Scopus subject areas

  • Medicine(all)
  • Cancer Research
  • Clinical Biochemistry
  • Molecular Medicine

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    Ziegler, K. M., Wade, T. E., Wang, S., Swartz-Basile, D. A., Pitt, H. A., & Zyromski, N. J. (2011). Validation of a novel, physiologic model of experimental acute pancreatitis in the mouse. American Journal of Translational Research, 3(2), 159-165.