Validation of urinary calcium isotope excretion from bone for screening anabolic therapies for osteoporosis

E. E. Hohman, G. P. McCabe, M. Peacock, C. M. Weaver

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Summary: Urinary excretion of calcium tracers in labeled individuals decreases in response to antiresorptive therapy, providing a tool to rapidly screen potential therapies. Using teriparatide, we demonstrate in this study that anabolic therapy also decreases tracer excretion, confirming that this method can also be used to screen potential anabolic therapies.

Introduction: Changes in urinary excretion of calcium tracers from a labeled skeleton may be a rapid and sensitive method to screen potential therapies for osteoporosis. This method has been used to screen antiresorptive therapies, but the effect of anabolic therapies on tracer excretion is unknown.

Methods: Eight-month-old female Sprague Dawley rats (n = 11) were given 50 μCi <sup>45</sup>Ca iv. After a 1-month equilibration period, baseline urinary <sup>45</sup>Ca excretion and total bone mineral content (BMC) were measured. Rats were then treated with 30 μg/kg teriparatide sc per day, a bone anabolic agent, for 80 days. Urine was collected throughout the study and analyzed for <sup>45</sup>Ca and total Ca, and BMC was measured at the beginning and end of the study.

Results: Teriparatide decreased urinary <sup>45</sup>Ca excretion by 52.1 % and increased BMC by 21.7 %. The change in bone calcium retention as determined by the ratio of <sup>45</sup>Ca to total Ca excretion in urine from day 6 through 15 of teriparatide treatment was significantly correlated (p = 0.036) with the change in BMC after 80 days of teriparatide treatment.

Conclusion: Urinary excretion of calcium tracers from labeled bone is an effective method to rapidly screen potential anabolic therapies for osteoporosis.

Original languageEnglish (US)
Pages (from-to)2471-2475
Number of pages5
JournalOsteoporosis International
Volume25
Issue number10
DOIs
StatePublished - Sep 26 2014

Keywords

  • Bone calcium label
  • Osteoporosis
  • Rats
  • Teriparatide
  • Urine calcium

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

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